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Detection and identification of chloramphenicol acetyltransferase gene in aeromonas isolates by multiplex polymerase chain reaction

A total of 37 Aeromonas isolates were isolated from retail fish in Malaysia wet market. In this study, the bacteria were tested for their haemolytic activity and susceptibility to antimicrobial agents. Twenty one (57) Aeromonas isolates showed positive haemolysin activity in this study. Six antimicr...

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Main Authors: Chua, K.H., Radu, S., Ismail, S., Hassan, E.H.
Format: Article
Published: 2005
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/3801/
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spelling my.um.eprints.38012014-10-20T05:37:31Z http://eprints.um.edu.my/3801/ Detection and identification of chloramphenicol acetyltransferase gene in aeromonas isolates by multiplex polymerase chain reaction Chua, K.H. Radu, S. Ismail, S. Hassan, E.H. R Medicine A total of 37 Aeromonas isolates were isolated from retail fish in Malaysia wet market. In this study, the bacteria were tested for their haemolytic activity and susceptibility to antimicrobial agents. Twenty one (57) Aeromonas isolates showed positive haemolysin activity in this study. Six antimicrobial agents were used in this study and all the Aeromonas isolates were found resistant to at least one antibiotic, and 15 of them (41) were multiresistant. Multiplex polymerase chain reaction was another approach used to detect the existence of chloramphenicol resistance gene in Aeromonas in this study. The chloramphenicol acetyltransferase (cat) gene was detected in 16 Aeromonas isolates. Nine of the Aeromonas isolates (56) carried cat II gene, while 1 (6), 2 (13), and 4 (25) of the remaining isolates carried cat I, cat III, and cat IV gene respectively. 2005 Article PeerReviewed Chua, K.H. and Radu, S. and Ismail, S. and Hassan, E.H. (2005) Detection and identification of chloramphenicol acetyltransferase gene in aeromonas isolates by multiplex polymerase chain reaction. Journal of Animal and Veterinary Advances, 4 (1). pp. 28-33. ISSN 1680-5593
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine
spellingShingle R Medicine
Chua, K.H.
Radu, S.
Ismail, S.
Hassan, E.H.
Detection and identification of chloramphenicol acetyltransferase gene in aeromonas isolates by multiplex polymerase chain reaction
description A total of 37 Aeromonas isolates were isolated from retail fish in Malaysia wet market. In this study, the bacteria were tested for their haemolytic activity and susceptibility to antimicrobial agents. Twenty one (57) Aeromonas isolates showed positive haemolysin activity in this study. Six antimicrobial agents were used in this study and all the Aeromonas isolates were found resistant to at least one antibiotic, and 15 of them (41) were multiresistant. Multiplex polymerase chain reaction was another approach used to detect the existence of chloramphenicol resistance gene in Aeromonas in this study. The chloramphenicol acetyltransferase (cat) gene was detected in 16 Aeromonas isolates. Nine of the Aeromonas isolates (56) carried cat II gene, while 1 (6), 2 (13), and 4 (25) of the remaining isolates carried cat I, cat III, and cat IV gene respectively.
format Article
author Chua, K.H.
Radu, S.
Ismail, S.
Hassan, E.H.
author_facet Chua, K.H.
Radu, S.
Ismail, S.
Hassan, E.H.
author_sort Chua, K.H.
title Detection and identification of chloramphenicol acetyltransferase gene in aeromonas isolates by multiplex polymerase chain reaction
title_short Detection and identification of chloramphenicol acetyltransferase gene in aeromonas isolates by multiplex polymerase chain reaction
title_full Detection and identification of chloramphenicol acetyltransferase gene in aeromonas isolates by multiplex polymerase chain reaction
title_fullStr Detection and identification of chloramphenicol acetyltransferase gene in aeromonas isolates by multiplex polymerase chain reaction
title_full_unstemmed Detection and identification of chloramphenicol acetyltransferase gene in aeromonas isolates by multiplex polymerase chain reaction
title_sort detection and identification of chloramphenicol acetyltransferase gene in aeromonas isolates by multiplex polymerase chain reaction
publishDate 2005
url http://eprints.um.edu.my/3801/
_version_ 1643687202468659200
score 13.209306

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