The pattern and frequency of t(14;18) translocation and immunophenotype in Asian follicular lymphoma

• Main Authors: Peh, S.C., Shaminie, J., Tai, Y.C., Tan, J.A.M.A., Gan, S.S.
• Format: Article
• Language: English
• Published: 2004
• Subjects: R Medicine
• Online Access: http://eprints.um.edu.my/3722/1/The_pattern_and_frequency_of_t%2814%3B18%29_translocation.pdf; http://eprints.um.edu.my/3722/

Aims: Follicular lymphoma is frequently associated with t(14;18)(q32;q21) translocation. This study was undertaken to determine the pattern of Bcl-2, CD10 and Bcl-6 expression in relation to t(14;18) translocation in follicular lymphoma from a cohort of a multi-ethnic Asian population. Methods and results: Sixty-two cases of follicular lymphoma were retrieved for immunohistochemistry, and t(14;18) translocation analysis by polymerase chain reaction and fluorescent in-situ hybridization techniques. Bcl-2 expression was present in 74 of the cases. CD10 expression was also relatively low (61), with decreasing frequency of expression in high-grade tumours. Bcl-6 protein was expressed in most of the tumours (88) regardless of the tumour grade. The t(14;18) translocation was detected in 46 cases (74) with an extremely high rate of t(14;18) translocation in ethnic Indian cases (100). Conclusion: The frequency of t(14;18) translocation in this series of follicular lymphomas was higher when compared with previous Asian reports, but in accordance with European and North American findings. CD10 expression is strongly associated with a t(14;18) translocation event, but the overall CD10 expression was relatively low, possibly due to the high proportion of high-grade tumours in the series. t(14;18) translocation was not associated with Bcl-2 or Bcl-6 expression.