Isolation and identification of inter-species enterovirus recombinant genomes
Positive-strand RNA virus evolution is partly attributed to the process of recombination. Although common between closely genetically related viruses, such as within species of the Enterovirus genus of the Picornaviridae family, inter-species recombination is rarely observed in nature. Recent studie...
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my.um.eprints.352802022-10-17T07:45:26Z http://eprints.um.edu.my/35280/ Isolation and identification of inter-species enterovirus recombinant genomes Bentley, Kirsten Tee, Han Kang Pearson, Ashley Lowry, Kym Waugh, Sheila Jones, Sian Chan, Yoke Fun Evans, David J. QR Microbiology QR355 Virology R Medicine Positive-strand RNA virus evolution is partly attributed to the process of recombination. Although common between closely genetically related viruses, such as within species of the Enterovirus genus of the Picornaviridae family, inter-species recombination is rarely observed in nature. Recent studies have shown recombination is a ubiquitous process, resulting in a wide range of recombinant genomes and progeny viruses. While not all recombinant genomes yield infectious progeny virus, their existence and continued evolution during replication have critical implications for the evolution of the virus population. In this study, we utilised an in vitro recombination assay to demonstrate inter-species recombination events between viruses from four enterovirus species, A-D. We show that inter-species recombinant genomes are generated in vitro with polymerase template-switching events occurring within the virus polyprotein coding region. However, these genomes did not yield infectious progeny virus. Analysis and attempted recovery of a constructed recombinant cDNA revealed a restriction in positive-strand but not negative-strand RNA synthesis, indicating a significant block in replication. This study demonstrates the propensity for inter-species recombination at the genome level but suggests that significant sequence plasticity would be required in order to overcome blocks in the virus life cycle and allow for the production of infectious viruses. MDPI 2021-12 Article PeerReviewed Bentley, Kirsten and Tee, Han Kang and Pearson, Ashley and Lowry, Kym and Waugh, Sheila and Jones, Sian and Chan, Yoke Fun and Evans, David J. (2021) Isolation and identification of inter-species enterovirus recombinant genomes. Viruses-Basel, 13 (12). ISSN 1999-4915, DOI https://doi.org/10.3390/v13122390 <https://doi.org/10.3390/v13122390>. 10.3390/v13122390 |
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QR Microbiology QR355 Virology R Medicine Bentley, Kirsten Tee, Han Kang Pearson, Ashley Lowry, Kym Waugh, Sheila Jones, Sian Chan, Yoke Fun Evans, David J. Isolation and identification of inter-species enterovirus recombinant genomes |
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Positive-strand RNA virus evolution is partly attributed to the process of recombination. Although common between closely genetically related viruses, such as within species of the Enterovirus genus of the Picornaviridae family, inter-species recombination is rarely observed in nature. Recent studies have shown recombination is a ubiquitous process, resulting in a wide range of recombinant genomes and progeny viruses. While not all recombinant genomes yield infectious progeny virus, their existence and continued evolution during replication have critical implications for the evolution of the virus population. In this study, we utilised an in vitro recombination assay to demonstrate inter-species recombination events between viruses from four enterovirus species, A-D. We show that inter-species recombinant genomes are generated in vitro with polymerase template-switching events occurring within the virus polyprotein coding region. However, these genomes did not yield infectious progeny virus. Analysis and attempted recovery of a constructed recombinant cDNA revealed a restriction in positive-strand but not negative-strand RNA synthesis, indicating a significant block in replication. This study demonstrates the propensity for inter-species recombination at the genome level but suggests that significant sequence plasticity would be required in order to overcome blocks in the virus life cycle and allow for the production of infectious viruses. |
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Article |
author |
Bentley, Kirsten Tee, Han Kang Pearson, Ashley Lowry, Kym Waugh, Sheila Jones, Sian Chan, Yoke Fun Evans, David J. |
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Bentley, Kirsten Tee, Han Kang Pearson, Ashley Lowry, Kym Waugh, Sheila Jones, Sian Chan, Yoke Fun Evans, David J. |
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Bentley, Kirsten |
title |
Isolation and identification of inter-species enterovirus recombinant genomes |
title_short |
Isolation and identification of inter-species enterovirus recombinant genomes |
title_full |
Isolation and identification of inter-species enterovirus recombinant genomes |
title_fullStr |
Isolation and identification of inter-species enterovirus recombinant genomes |
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Isolation and identification of inter-species enterovirus recombinant genomes |
title_sort |
isolation and identification of inter-species enterovirus recombinant genomes |
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MDPI |
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2021 |
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http://eprints.um.edu.my/35280/ |
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1748181070375813120 |
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13.18916 |