A 3D microfluidic elisa for the detection of severe dengue: sensitivity improvement and vroman effect amelioration by edc-nhs surface modification

• Main Authors: Maeno, Hinata, Wong, Pooi-Fong, AbuBakar, Sazaly, Yang, Ming, Sam, Sing-Sin, Jamil-Abd, Juraina, Shunmugarajoo, Anusha, Mustafa, Mahiran, Said, Rosaida Md, Mageswaren, Eashwary, Azmel, Azureen, Mat Jelani, Anilawati
• Format: Article
• Published: MDPI 2021
• Subjects: Q Science (General); QD Chemistry
• Online Access: http://eprints.um.edu.my/34535/

Serum is commonly used as a specimen in immunoassays but the presence of heterophilic antibodies can potentially interfere with the test results. Previously, we have developed a microfluidic device called: 3D Stack for enzyme-linked immunosorbent assay (ELISA). However, its evaluation was limited to...