Sera of IgA nephropathy patients contain a heterogeneous population of relatively cationic alpha-heavy chains

• Main Authors: Shuib, A.S., Chua, C.T., Hashim, Onn Haji
• Format: Article
• Language: English
• Published: 1998
• Subjects: R Medicine
• Online Access: http://eprints.um.edu.my/3446/1/Sera_of_IgA_nephropathy_patients_contain_a_heterogeneous_population_of_relatively_cationic_alpha-heavy_chains.pdf; http://eprints.um.edu.my/3446/; http://content.karger.com/ProdukteDB/produkte.asp?doi=10.1159/000044938

Sera of IgA nephropathy (IgAN) patients and normal subjects were analysed by two-dimensional (2-D) gel electrophoresis. Densitometric analysis of the 2-D gels of IgAN patients and normal subjects revealed that their protein maps were comparable. There was no shift of pI values in the major alpha-heavy chain spots. However, the volume of the alpha-heavy chain bands were differently distributed. Distribution was significantly lower at the anionic region in IgAN patients (mean anionic:cationic ratio of 1.184 +/- 0.311) as compared to normal healthy controls (mean anionic:cationic ratio of 2.139 +/- 0.538). Our data are in support of the previously reported findings that IgA1 of IgAN patients were lacking in sialic acid residues.