Actinomadura graeca sp. nov.: A novel producer of the macrocyclic antibiotic zelkovamycin

As part of a screening programme for antibiotic-producing bacteria, a novel Actinomadura species was discovered from a soil sample collected in Santorini, Greece. Preliminary 16S rRNA gene sequence comparisons highlighted Actinomadura macra as the most similar characterised species. However, whole-g...

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Main Authors: Tarantini, Francesco Saverio, Brunati, Mara, Taravella, Anna, Carrano, Lucia, Parenti, Francesco, Hong, Kar Wai, Williams, Paul, Chan, Kok Gan, Heeb, Stephan, Chan, Weng C.
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Published: Public Library of Science 2021
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Online Access:http://eprints.um.edu.my/34390/
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spelling my.um.eprints.343902022-06-09T03:47:04Z http://eprints.um.edu.my/34390/ Actinomadura graeca sp. nov.: A novel producer of the macrocyclic antibiotic zelkovamycin Tarantini, Francesco Saverio Brunati, Mara Taravella, Anna Carrano, Lucia Parenti, Francesco Hong, Kar Wai Williams, Paul Chan, Kok Gan Heeb, Stephan Chan, Weng C. Q Science (General) As part of a screening programme for antibiotic-producing bacteria, a novel Actinomadura species was discovered from a soil sample collected in Santorini, Greece. Preliminary 16S rRNA gene sequence comparisons highlighted Actinomadura macra as the most similar characterised species. However, whole-genome sequencing revealed an average nucleotide identity (ANI) value of 89% with A. macra, the highest among related species. Further phenotypic and chemotaxonomic analyses confirmed that the isolate represents a previously uncharacterised species in the genus Actinomadura, for which the name Actinomadura graeca sp. nov. is proposed (type strain 32-07(T)). The G+C content of A. graeca 32-07 is 72.36%. The cell wall contains DL-diaminopimelic acid, intracellular sugars are glucose, ribose and galactose, the predominant menaquinone is MK-9(H-6), the major cellular lipid is phosphatidylinositol and fatty acids consist mainly of hexadecanoic acid. No mycolic acid was detected. Furthermore, A. graeca 32-07 has been confirmed as a novel producer of the non-ribosomal peptide antibiotic zelkovamycin and we report herein a provisional description of the unique biosynthetic gene cluster. Public Library of Science 2021-11-30 Article PeerReviewed Tarantini, Francesco Saverio and Brunati, Mara and Taravella, Anna and Carrano, Lucia and Parenti, Francesco and Hong, Kar Wai and Williams, Paul and Chan, Kok Gan and Heeb, Stephan and Chan, Weng C. (2021) Actinomadura graeca sp. nov.: A novel producer of the macrocyclic antibiotic zelkovamycin. PLoS ONE, 16 (11). ISSN 1932-6203, DOI https://doi.org/10.1371/journal.pone.0260413 <https://doi.org/10.1371/journal.pone.0260413>. 10.1371/journal.pone.0260413
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic Q Science (General)
spellingShingle Q Science (General)
Tarantini, Francesco Saverio
Brunati, Mara
Taravella, Anna
Carrano, Lucia
Parenti, Francesco
Hong, Kar Wai
Williams, Paul
Chan, Kok Gan
Heeb, Stephan
Chan, Weng C.
Actinomadura graeca sp. nov.: A novel producer of the macrocyclic antibiotic zelkovamycin
description As part of a screening programme for antibiotic-producing bacteria, a novel Actinomadura species was discovered from a soil sample collected in Santorini, Greece. Preliminary 16S rRNA gene sequence comparisons highlighted Actinomadura macra as the most similar characterised species. However, whole-genome sequencing revealed an average nucleotide identity (ANI) value of 89% with A. macra, the highest among related species. Further phenotypic and chemotaxonomic analyses confirmed that the isolate represents a previously uncharacterised species in the genus Actinomadura, for which the name Actinomadura graeca sp. nov. is proposed (type strain 32-07(T)). The G+C content of A. graeca 32-07 is 72.36%. The cell wall contains DL-diaminopimelic acid, intracellular sugars are glucose, ribose and galactose, the predominant menaquinone is MK-9(H-6), the major cellular lipid is phosphatidylinositol and fatty acids consist mainly of hexadecanoic acid. No mycolic acid was detected. Furthermore, A. graeca 32-07 has been confirmed as a novel producer of the non-ribosomal peptide antibiotic zelkovamycin and we report herein a provisional description of the unique biosynthetic gene cluster.
format Article
author Tarantini, Francesco Saverio
Brunati, Mara
Taravella, Anna
Carrano, Lucia
Parenti, Francesco
Hong, Kar Wai
Williams, Paul
Chan, Kok Gan
Heeb, Stephan
Chan, Weng C.
author_facet Tarantini, Francesco Saverio
Brunati, Mara
Taravella, Anna
Carrano, Lucia
Parenti, Francesco
Hong, Kar Wai
Williams, Paul
Chan, Kok Gan
Heeb, Stephan
Chan, Weng C.
author_sort Tarantini, Francesco Saverio
title Actinomadura graeca sp. nov.: A novel producer of the macrocyclic antibiotic zelkovamycin
title_short Actinomadura graeca sp. nov.: A novel producer of the macrocyclic antibiotic zelkovamycin
title_full Actinomadura graeca sp. nov.: A novel producer of the macrocyclic antibiotic zelkovamycin
title_fullStr Actinomadura graeca sp. nov.: A novel producer of the macrocyclic antibiotic zelkovamycin
title_full_unstemmed Actinomadura graeca sp. nov.: A novel producer of the macrocyclic antibiotic zelkovamycin
title_sort actinomadura graeca sp. nov.: a novel producer of the macrocyclic antibiotic zelkovamycin
publisher Public Library of Science
publishDate 2021
url http://eprints.um.edu.my/34390/
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score 13.209306