Cordycepin inhibits virus replication in dengue virus-infected vero cells
Dengue virus (DENV) infection causes mild to severe illness in humans that can lead to fatality in severe cases. Currently, no specific drug is available for the treatment of DENV infection. Thus, the development of an anti-DENV drug is urgently required. Cordycepin (3 `-deoxyadenosine), which is a...
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my.um.eprints.343782022-06-09T04:21:03Z http://eprints.um.edu.my/34378/ Cordycepin inhibits virus replication in dengue virus-infected vero cells Panya, Aussara Songprakhon, Pucharee Panwong, Suthida Jantakee, Kanyaluck Kaewkod, Thida Tragoolpua, Yingmanee Sawasdee, Nunghathai Lee, Vannajan Sanghiran Nimmanpipug, Piyarat Yenchitsomanus, Pa-thai QD Chemistry QH301 Biology Dengue virus (DENV) infection causes mild to severe illness in humans that can lead to fatality in severe cases. Currently, no specific drug is available for the treatment of DENV infection. Thus, the development of an anti-DENV drug is urgently required. Cordycepin (3 `-deoxyadenosine), which is a major bioactive compound in Cordyceps (ascomycete) fungus that has been used for centuries in Chinese traditional medicine, was reported to exhibit antiviral activity. However, the anti-DENV activity of cordycepin is unknown. We hypothesized that cordycepin exerts anti-DENV activity and that, as an adenosine derivative, it inhibits DENV replication. To test this hypothesis, we investigated the anti-DENV activity of cordycepin in DENV-infected Vero cells. Cordycepin treatment significantly decreased DENV protein at a half-maximal effective concentration (EC50) of 26.94 mu M. Moreover, DENV RNA was dramatically decreased in cordycepin-treated Vero cells, indicating its effectiveness in inhibiting viral RNA replication. Via in silico molecular docking, the binding of cordycepin to DENV non-structural protein 5 (NS5), which is an important enzyme for RNA synthesis, at both the methyltransferase (MTase) and RNA-dependent RNA polymerase (RdRp) domains, was predicted. The results of this study demonstrate that cordycepin is able to inhibit DENV replication, which portends its potential as an anti-dengue therapy. MDPI 2021-06 Article PeerReviewed Panya, Aussara and Songprakhon, Pucharee and Panwong, Suthida and Jantakee, Kanyaluck and Kaewkod, Thida and Tragoolpua, Yingmanee and Sawasdee, Nunghathai and Lee, Vannajan Sanghiran and Nimmanpipug, Piyarat and Yenchitsomanus, Pa-thai (2021) Cordycepin inhibits virus replication in dengue virus-infected vero cells. Molecules, 26 (11). ISSN 1420-3049, DOI https://doi.org/10.3390/molecules26113118 <https://doi.org/10.3390/molecules26113118>. 10.3390/molecules26113118 |
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QD Chemistry QH301 Biology Panya, Aussara Songprakhon, Pucharee Panwong, Suthida Jantakee, Kanyaluck Kaewkod, Thida Tragoolpua, Yingmanee Sawasdee, Nunghathai Lee, Vannajan Sanghiran Nimmanpipug, Piyarat Yenchitsomanus, Pa-thai Cordycepin inhibits virus replication in dengue virus-infected vero cells |
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Dengue virus (DENV) infection causes mild to severe illness in humans that can lead to fatality in severe cases. Currently, no specific drug is available for the treatment of DENV infection. Thus, the development of an anti-DENV drug is urgently required. Cordycepin (3 `-deoxyadenosine), which is a major bioactive compound in Cordyceps (ascomycete) fungus that has been used for centuries in Chinese traditional medicine, was reported to exhibit antiviral activity. However, the anti-DENV activity of cordycepin is unknown. We hypothesized that cordycepin exerts anti-DENV activity and that, as an adenosine derivative, it inhibits DENV replication. To test this hypothesis, we investigated the anti-DENV activity of cordycepin in DENV-infected Vero cells. Cordycepin treatment significantly decreased DENV protein at a half-maximal effective concentration (EC50) of 26.94 mu M. Moreover, DENV RNA was dramatically decreased in cordycepin-treated Vero cells, indicating its effectiveness in inhibiting viral RNA replication. Via in silico molecular docking, the binding of cordycepin to DENV non-structural protein 5 (NS5), which is an important enzyme for RNA synthesis, at both the methyltransferase (MTase) and RNA-dependent RNA polymerase (RdRp) domains, was predicted. The results of this study demonstrate that cordycepin is able to inhibit DENV replication, which portends its potential as an anti-dengue therapy. |
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Article |
author |
Panya, Aussara Songprakhon, Pucharee Panwong, Suthida Jantakee, Kanyaluck Kaewkod, Thida Tragoolpua, Yingmanee Sawasdee, Nunghathai Lee, Vannajan Sanghiran Nimmanpipug, Piyarat Yenchitsomanus, Pa-thai |
author_facet |
Panya, Aussara Songprakhon, Pucharee Panwong, Suthida Jantakee, Kanyaluck Kaewkod, Thida Tragoolpua, Yingmanee Sawasdee, Nunghathai Lee, Vannajan Sanghiran Nimmanpipug, Piyarat Yenchitsomanus, Pa-thai |
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Panya, Aussara |
title |
Cordycepin inhibits virus replication in dengue virus-infected vero cells |
title_short |
Cordycepin inhibits virus replication in dengue virus-infected vero cells |
title_full |
Cordycepin inhibits virus replication in dengue virus-infected vero cells |
title_fullStr |
Cordycepin inhibits virus replication in dengue virus-infected vero cells |
title_full_unstemmed |
Cordycepin inhibits virus replication in dengue virus-infected vero cells |
title_sort |
cordycepin inhibits virus replication in dengue virus-infected vero cells |
publisher |
MDPI |
publishDate |
2021 |
url |
http://eprints.um.edu.my/34378/ |
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1735570303484952576 |
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13.160551 |