Identifying kinase targets of PPAR gamma in human breast cancer

• Main Authors: Kandel, Anish, Dhillon, Sarinder Kaur Kashmir Singh, Prabaharan, Chandra Bose, Hisham, Syaza Fatnin Binti, Rajamanickam, Karthic, Napper, Scott, Chidambaram, Saravana Babu, Essa, Musthafa Mohamed, Yang, Jian, Sakharkar, Meena Kishore
• Format: Article
• Published: Taylor & Francis Ltd 2021
• Subjects: R Medicine; RC Internal medicine; RC0254 Neoplasms. Tumors. Oncology (including Cancer)
• Online Access: http://eprints.um.edu.my/34357/

Breast cancer is the most common cancer in women. Despite advances in screening women for genetic predisposition to breast cancer and risk stratification, a majority of women carriers remain undetected until they become affected. Thus, there is a need to develop a cost-effective, rapid, sensitive and non-invasive early-stage diagnostic method. Kinases are involved in all fundamental cellular processes and mutations in kinases have been reported as drivers of cancer. PPAR gamma is a ligand-activated transcription factor that plays important roles in cell proliferation and metabolism. However, the complete set of kinases modulated by PPAR gamma is still unknown. In this study, we identified human kinases that are potential PPAR gamma targets and evaluated their differential expression and gene pair correlations in human breast cancer patient dataset TCGA-BRCA. We further confirmed the findings in human breast cancer cell lines MCF7 and SK-BR-3 using a kinome array. We observed that gene pair correlations are lost in tumours as compared to healthy controls and could be used as a supplement strategy for diagnosis and prognosis of breast cancer.