Identification of immunogenic proteins from Burkholderia cepacia secretome using proteomic analysis
Burkholderia cepacia is an opportunistic human pathogen associated with lung infections. Secretory proteins of B. cepacia are known to be involved in virulence and may mediate important host-pathogen interactions. In the present study, secretory proteins isolated from B. cepacia culture supernatant...
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my.um.eprints.34332019-10-24T08:25:22Z http://eprints.um.edu.my/3433/ Identification of immunogenic proteins from Burkholderia cepacia secretome using proteomic analysis Mariappan, V. Vellasamy, K.M. Thimma, J.S. Hashim, Onn Haji Vadivelu, J. R Medicine Burkholderia cepacia is an opportunistic human pathogen associated with lung infections. Secretory proteins of B. cepacia are known to be involved in virulence and may mediate important host-pathogen interactions. In the present study, secretory proteins isolated from B. cepacia culture supernatant were separated using two-dimensional gel electrophoresis, followed by Western blot analysis to identify the immunogenic proteins. Mice antibodies raised to B. cepacia inactivated whole bacteria, outer membrane protein and culture filtrate antigen detected 74,104 and 32 immunogenic proteins, respectively. Eighteen of these immunogenic proteins which reacted with all three antibodies were identified and might be potential molecules as a diagnostic marker or a putative candidate vaccine against B. cepacia infections. (C) 2009 Elsevier Ltd. All rights reserved. 2010 Article PeerReviewed application/pdf en http://eprints.um.edu.my/3433/1/Identification_of_immunogenic_proteins_from_Burkholderia_cepacia_secretome_using_proteomic_analysis.pdf Mariappan, V. and Vellasamy, K.M. and Thimma, J.S. and Hashim, Onn Haji and Vadivelu, J. (2010) Identification of immunogenic proteins from Burkholderia cepacia secretome using proteomic analysis. Vaccine, 28 (5). pp. 1318-1324. ISSN 0264-410X http://www.sciencedirect.com/science/article/pii/S0264410X09017915 10.1016/j.vaccine.2009.11.027 |
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Burkholderia cepacia is an opportunistic human pathogen associated with lung infections. Secretory proteins of B. cepacia are known to be involved in virulence and may mediate important host-pathogen interactions. In the present study, secretory proteins isolated from B. cepacia culture supernatant were separated using two-dimensional gel electrophoresis, followed by Western blot analysis to identify the immunogenic proteins. Mice antibodies raised to B. cepacia inactivated whole bacteria, outer membrane protein and culture filtrate antigen detected 74,104 and 32 immunogenic proteins, respectively. Eighteen of these immunogenic proteins which reacted with all three antibodies were identified and might be potential molecules as a diagnostic marker or a putative candidate vaccine against B. cepacia infections. (C) 2009 Elsevier Ltd. All rights reserved. |
format |
Article |
author |
Mariappan, V. Vellasamy, K.M. Thimma, J.S. Hashim, Onn Haji Vadivelu, J. |
author_facet |
Mariappan, V. Vellasamy, K.M. Thimma, J.S. Hashim, Onn Haji Vadivelu, J. |
author_sort |
Mariappan, V. |
title |
Identification of immunogenic proteins from Burkholderia cepacia secretome using proteomic analysis |
title_short |
Identification of immunogenic proteins from Burkholderia cepacia secretome using proteomic analysis |
title_full |
Identification of immunogenic proteins from Burkholderia cepacia secretome using proteomic analysis |
title_fullStr |
Identification of immunogenic proteins from Burkholderia cepacia secretome using proteomic analysis |
title_full_unstemmed |
Identification of immunogenic proteins from Burkholderia cepacia secretome using proteomic analysis |
title_sort |
identification of immunogenic proteins from burkholderia cepacia secretome using proteomic analysis |
publishDate |
2010 |
url |
http://eprints.um.edu.my/3433/1/Identification_of_immunogenic_proteins_from_Burkholderia_cepacia_secretome_using_proteomic_analysis.pdf http://eprints.um.edu.my/3433/ http://www.sciencedirect.com/science/article/pii/S0264410X09017915 |
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