Molecular characterization of Giardia duodenalis isolated from Semai Pahang Orang Asli (peninsular Malaysia aborigines)

• Main Authors: Mahdy, A.K.M., Surin, J., Mohd-Adnan, A., Wan, K.L., Lim, Y.A.L.
• Format: Article
• Language: English
• Published: 2009
• Subjects: R Medicine
• Online Access: http://eprints.um.edu.my/3360/1/Molecular_characterization_of_Giardia_duodenalis_isolated.pdf; http://eprints.um.edu.my/3360/

This study was conducted to determine the genotypes of Giardia duodenalis isolated from human faecal samples at Pos Betau, Pahang, Malaysia. Faecal specimens were collected and examined for G. aduodenalis cysts using Trichrome staining techniques. Molecular identification was carried Out by the amplification of a region of the small subunit of the nuclear ribosomal RNA (SSU rRNA) gene using nested PCR and Subsequent sequencing. The sequences front 15 isolates from G. duodenalis were subjected to phylogenetic analysis (including appropriate outgroups) using the neighbor-joining and maximum parsimony methods. The trees identified G. duodenalis assemblages A and 13, with a predominance of assemblage B. The predominance of anthroponotic genotypes indicates the possibility of anthroponotic transmission of these protozoa in this Semai Pahang Orang Ash community.