A preliminary study of human amniotic membrane as a potential chondrocyte carrier
Purpose: To investigate the feasibility of using processed human amniotic membrane (HAM) to support the attachment and proliferation of chondrocytes in vitro which in turn can be utilised as a cell delivery vehicle in tissue engineering applications. Methods: Fresh HAM obtained from patients undergo...
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University of Malaya
2009
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Online Access: | http://eprints.um.edu.my/3215/1/A_Preliminary_Study_of_Human_Amniotic_Membrane_as_a_Potential_Chondrocyte_Carrier.pdf http://eprints.um.edu.my/3215/ http://www.morthoj.org/2009v3n2/Chondrocyte-Carrier.pdf |
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my.um.eprints.32152020-02-10T08:33:38Z http://eprints.um.edu.my/3215/ A preliminary study of human amniotic membrane as a potential chondrocyte carrier Boo, L. Sofiah, S. Selvaratnam, L. Tai, C.C. Pingguan-Murphy, Belinda Kamarul, Tunku R Medicine Purpose: To investigate the feasibility of using processed human amniotic membrane (HAM) to support the attachment and proliferation of chondrocytes in vitro which in turn can be utilised as a cell delivery vehicle in tissue engineering applications. Methods: Fresh HAM obtained from patients undergoing routine elective caesarean sections was harvested, processed and dried using either freeze drying (FD) or air drying (AD) methods prior to sterilisation by gamma irradiation. Isolated, processed and characterised rabbit autologous chondrocytes were seeded on processed HAM and cultured for up to three weeks. Cell attachment and proliferation were examined qualitatively using inverted brightfield microscopy. Results: Processed HAM appeared to allow cell attachment when implanted with chondrocytes. Although cells seeded on AD and FD HAM did not appear to attach as strongly as those seeded on glycerol preserved intact human amniotic membrane, these cells to be proliferated in cell culture conditions. Conclusion: Preliminary results show that processed HAM promotes chondrocyte attachment and proliferation. University of Malaya 2009 Article PeerReviewed application/pdf en http://eprints.um.edu.my/3215/1/A_Preliminary_Study_of_Human_Amniotic_Membrane_as_a_Potential_Chondrocyte_Carrier.pdf Boo, L. and Sofiah, S. and Selvaratnam, L. and Tai, C.C. and Pingguan-Murphy, Belinda and Kamarul, Tunku (2009) A preliminary study of human amniotic membrane as a potential chondrocyte carrier. Malaysian Orthopaedic Journal, 3 (2). pp. 16-23. ISSN 1985-2533 http://www.morthoj.org/2009v3n2/Chondrocyte-Carrier.pdf |
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Purpose: To investigate the feasibility of using processed human amniotic membrane (HAM) to support the attachment and proliferation of chondrocytes in vitro which in turn can be utilised as a cell delivery vehicle in tissue engineering applications. Methods: Fresh HAM obtained from patients undergoing routine elective caesarean sections was harvested, processed and dried using either freeze drying (FD) or air drying (AD) methods prior to sterilisation by gamma irradiation. Isolated, processed and characterised rabbit autologous chondrocytes were seeded on processed HAM and cultured for up to three weeks. Cell attachment and proliferation were examined qualitatively using inverted brightfield microscopy. Results: Processed HAM appeared to allow cell attachment when implanted with chondrocytes. Although cells seeded on AD and FD HAM did not appear to attach as strongly as those seeded on glycerol preserved intact human amniotic membrane, these cells to be proliferated in cell culture conditions. Conclusion: Preliminary results show that processed HAM promotes chondrocyte attachment and proliferation. |
format |
Article |
author |
Boo, L. Sofiah, S. Selvaratnam, L. Tai, C.C. Pingguan-Murphy, Belinda Kamarul, Tunku |
author_facet |
Boo, L. Sofiah, S. Selvaratnam, L. Tai, C.C. Pingguan-Murphy, Belinda Kamarul, Tunku |
author_sort |
Boo, L. |
title |
A preliminary study of human amniotic membrane as a potential chondrocyte carrier |
title_short |
A preliminary study of human amniotic membrane as a potential chondrocyte carrier |
title_full |
A preliminary study of human amniotic membrane as a potential chondrocyte carrier |
title_fullStr |
A preliminary study of human amniotic membrane as a potential chondrocyte carrier |
title_full_unstemmed |
A preliminary study of human amniotic membrane as a potential chondrocyte carrier |
title_sort |
preliminary study of human amniotic membrane as a potential chondrocyte carrier |
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University of Malaya |
publishDate |
2009 |
url |
http://eprints.um.edu.my/3215/1/A_Preliminary_Study_of_Human_Amniotic_Membrane_as_a_Potential_Chondrocyte_Carrier.pdf http://eprints.um.edu.my/3215/ http://www.morthoj.org/2009v3n2/Chondrocyte-Carrier.pdf |
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