Pseudocyst forms of Trichomonas vaginalis from cervical neoplasia

Trichomonas vaginalis, a flagellated protozoan parasite causes a variety of adverse health consequences in both men and women. The parasite exists in the trophozoite and the pseudocystic stage. The study reports for the first time that pseudocyst forms of T. vaginalis isolated from cervical neoplasi...

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Main Authors: Afzan, M.Y., Suresh, K.
Format: Article
Published: Springer Verlag (Germany) 2012
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Online Access:http://eprints.um.edu.my/2911/
http://www.ncbi.nlm.nih.gov/pubmed/22398830
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spelling my.um.eprints.29112013-11-11T02:14:58Z http://eprints.um.edu.my/2911/ Pseudocyst forms of Trichomonas vaginalis from cervical neoplasia Afzan, M.Y. Suresh, K. R Medicine Trichomonas vaginalis, a flagellated protozoan parasite causes a variety of adverse health consequences in both men and women. The parasite exists in the trophozoite and the pseudocystic stage. The study reports for the first time that pseudocyst forms of T. vaginalis isolated from cervical neoplasia (CN) patients demonstrated distinct, different and significant in vitro growth profiles when grown in vitro cultures from day 1 up to day 5 (p < 0.05, Mann-Whitney test) when compared with the same life cycle stages isolated from non-cervical neoplasia but symptomatic patients (NCN). Pseudocysts from CN and NCN isolates remained viable in distilled water until 3 h 10 min and 2 h 10 min, respectively. The nucleus of pseudocysts in CN isolates using acridine orange and DAPI showed more intense staining revealing higher nuclear content. The FITC-labeled Concanavalin A stained stronger green fluorescence with surface of pseudocysts in CN isolates showing more rough and creased surface with higher numbers of deep micropores with larger numbers of chromatin masses, vacuoles, and hydrogenosomes. The study confirms that pseudocystic stage from CN, despite the uniformity in appearance of being rounded and showing no motility without a true cyst wall under light microscopy, demonstrated different biochemical, surface, and ultrastructural properties. The study provides evidence that phenotypic variant forms of pseudocysts does exist and possibly does play a role in exacerbating cervical cancer. Springer Verlag (Germany) 2012-03 Article PeerReviewed Afzan, M.Y. and Suresh, K. (2012) Pseudocyst forms of Trichomonas vaginalis from cervical neoplasia. Parasitology Research. ISSN 0932-0113 http://www.ncbi.nlm.nih.gov/pubmed/22398830 PMID: 22398830
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine
spellingShingle R Medicine
Afzan, M.Y.
Suresh, K.
Pseudocyst forms of Trichomonas vaginalis from cervical neoplasia
description Trichomonas vaginalis, a flagellated protozoan parasite causes a variety of adverse health consequences in both men and women. The parasite exists in the trophozoite and the pseudocystic stage. The study reports for the first time that pseudocyst forms of T. vaginalis isolated from cervical neoplasia (CN) patients demonstrated distinct, different and significant in vitro growth profiles when grown in vitro cultures from day 1 up to day 5 (p < 0.05, Mann-Whitney test) when compared with the same life cycle stages isolated from non-cervical neoplasia but symptomatic patients (NCN). Pseudocysts from CN and NCN isolates remained viable in distilled water until 3 h 10 min and 2 h 10 min, respectively. The nucleus of pseudocysts in CN isolates using acridine orange and DAPI showed more intense staining revealing higher nuclear content. The FITC-labeled Concanavalin A stained stronger green fluorescence with surface of pseudocysts in CN isolates showing more rough and creased surface with higher numbers of deep micropores with larger numbers of chromatin masses, vacuoles, and hydrogenosomes. The study confirms that pseudocystic stage from CN, despite the uniformity in appearance of being rounded and showing no motility without a true cyst wall under light microscopy, demonstrated different biochemical, surface, and ultrastructural properties. The study provides evidence that phenotypic variant forms of pseudocysts does exist and possibly does play a role in exacerbating cervical cancer.
format Article
author Afzan, M.Y.
Suresh, K.
author_facet Afzan, M.Y.
Suresh, K.
author_sort Afzan, M.Y.
title Pseudocyst forms of Trichomonas vaginalis from cervical neoplasia
title_short Pseudocyst forms of Trichomonas vaginalis from cervical neoplasia
title_full Pseudocyst forms of Trichomonas vaginalis from cervical neoplasia
title_fullStr Pseudocyst forms of Trichomonas vaginalis from cervical neoplasia
title_full_unstemmed Pseudocyst forms of Trichomonas vaginalis from cervical neoplasia
title_sort pseudocyst forms of trichomonas vaginalis from cervical neoplasia
publisher Springer Verlag (Germany)
publishDate 2012
url http://eprints.um.edu.my/2911/
http://www.ncbi.nlm.nih.gov/pubmed/22398830
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