Real-time loop-mediated isothermal amplification assay for rapid detection of human papillomavirus 16 in oral squamous cell carcinoma

• Main Authors: Hamzan, Nurul Izzati, Ab Rahman, Nurhayu, Suraiya, Siti, Mohamad, Irfan, Kalarakkal, Thomas George, Mohamad, Suharni
• Format: Article
• Published: Elsevier 2021
• Subjects: R Medicine (General); RK Dentistry
• Online Access: http://eprints.um.edu.my/28423/

Objective: The present study established a real-time loop-mediated isothermal amplification (qLAMP) for rapid detection of human papillomavirus subtype 16 (HPV-16) in oral squamous cell carcinoma (OSCC). Methods: The qLAMP assay was optimized targeting the HPV-16 E7 gene. The analytical sensitivity...