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Optimization for high-level expression in pichia pastoris and purification of truncated and full length recombinant SAG2 of toxoplasma gondii for diagnostic use

SAG2 is one of the major surface antigens of the intracellular protozoan parasite Toxoplasma gondii. In the present study, truncated recombinant SAG2(S) and full length recombinant SAG2(T) of T. gondii were optimally produced (similar to 15 mg/liter) in Pichia pus tons expression system using BMMY m...

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Bibliographic Details
Main Authors: Ling, L.Y., Ithoi, Init, Fong, Mun Yik
Format: Article
Language:English
Published: SEAMEO Regional Tropical Medicine and Public Health Network 2010
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/2731/1/OPTIMIZATION_FOR_HIGH-LEVEL_EXPRESSION_IN_PICHIA_PASTORIS_AND_PURIFICATION_OF_TRUNCATED_AND_FULL_LENGTH_RECOMBINANT_SAG2_OF_TOXOPLASMA_GONDII_FOR_DIAGNOSTIC_USE.pdf
http://eprints.um.edu.my/2731/
http://www.tm.mahidol.ac.th/seameo/2010-41-3/01-4781.pdf
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http://eprints.um.edu.my/2731/1/OPTIMIZATION_FOR_HIGH-LEVEL_EXPRESSION_IN_PICHIA_PASTORIS_AND_PURIFICATION_OF_TRUNCATED_AND_FULL_LENGTH_RECOMBINANT_SAG2_OF_TOXOPLASMA_GONDII_FOR_DIAGNOSTIC_USE.pdf
http://eprints.um.edu.my/2731/
http://www.tm.mahidol.ac.th/seameo/2010-41-3/01-4781.pdf

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