Recombinant expression of the larval excretory-secretory antigen TES-120 of Toxocara canis in the methylotrophic yeast Pichia pastoris
A gene encoding the larval excretory-secretory antigen TES-120 of the dog ascarid worm Toxocara canis was cloned into the methylotrophic yeast Pichia pastoris. Specificity of the recombinant TES-120 antigen produced by the yeast was investigated. Forty-five human serum samples from patients infected...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Springer Verlag (Germany)
2004
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| Subjects: | |
| Online Access: | http://eprints.um.edu.my/2702/1/Recombinant_expression_of_the_larval_excretory-secretory_antigen_TES-120_of_Toxocara_canis_in_the_methylotrophic_yeast_Pichia_pastoris.pdf http://eprints.um.edu.my/2702/ http://www.springerlink.com/content/69qph4ld2ub9vvjd/ |
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| Summary: | A gene encoding the larval excretory-secretory antigen TES-120 of the dog ascarid worm Toxocara canis was cloned into the methylotrophic yeast Pichia pastoris. Specificity of the recombinant TES-120 antigen produced by the yeast was investigated. Forty-five human serum samples from patients infected with differentparasitic organisms, including 8 cases of toxocariasis, were tested against the recombinant antigen in immunoblot assays. Results from the assays showed that the recombinant TES-120 antigen reacted with sera from toxocariasis patients only. This highly specific recombinant TES-120 antigen can potentially be used for the development of an inexpensive serodiagnostic assay for human toxocariasis. |
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