CRISPR/dCas9-based systems: Mechanisms and applications in plant sciences
RNA-guided genomic transcriptional regulation tools, namely clustered regularly interspaced short palindromic repeats interference (CRISPRi) and CRISPR-mediated gene activation (CRISPRa), are a powerful technology for gene functional studies. Deriving from the CRISPR/Cas9 system, both systems consis...
Saved in:
Main Authors: | , , , |
---|---|
Format: | Article |
Published: |
MDPI
2021
|
Subjects: | |
Online Access: | http://eprints.um.edu.my/26413/ |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
my.um.eprints.26413 |
---|---|
record_format |
eprints |
spelling |
my.um.eprints.264132022-02-28T01:26:12Z http://eprints.um.edu.my/26413/ CRISPR/dCas9-based systems: Mechanisms and applications in plant sciences Karlson, Chou Khai Soong Mohd-Noor, Siti Nurfadhlina Nolte, Nadja Tan, Boon Chin Q Science (General) RNA-guided genomic transcriptional regulation tools, namely clustered regularly interspaced short palindromic repeats interference (CRISPRi) and CRISPR-mediated gene activation (CRISPRa), are a powerful technology for gene functional studies. Deriving from the CRISPR/Cas9 system, both systems consist of a catalytically dead Cas9 (dCas9), a transcriptional effector and a single guide RNA (sgRNA). This type of dCas9 is incapable to cleave DNA but retains its ability to specifically bind to DNA. The binding of the dCas9/sgRNA complex to a target gene results in transcriptional interference. The CRISPR/dCas9 system has been explored as a tool for transcriptional modulation and genome imaging. Despite its potential applications and benefits, the challenges and limitations faced by the CRISPR/dCas9 system include the off-target effects, protospacer adjacent motif (PAM) sequence requirements, efficient delivery methods and the CRISPR/dCas9-interfered crops being labeled as genetically modified organisms in several countries. This review highlights the progression of CRISPR/dCas9 technology as well as its applications and potential challenges in crop improvement.</p> MDPI 2021-10 Article PeerReviewed Karlson, Chou Khai Soong and Mohd-Noor, Siti Nurfadhlina and Nolte, Nadja and Tan, Boon Chin (2021) CRISPR/dCas9-based systems: Mechanisms and applications in plant sciences. Plants-Basel, 10 (10). ISSN 2223-7747, DOI https://doi.org/10.3390/plants10102055 <https://doi.org/10.3390/plants10102055>. 10.3390/plants10102055 |
institution |
Universiti Malaya |
building |
UM Library |
collection |
Institutional Repository |
continent |
Asia |
country |
Malaysia |
content_provider |
Universiti Malaya |
content_source |
UM Research Repository |
url_provider |
http://eprints.um.edu.my/ |
topic |
Q Science (General) |
spellingShingle |
Q Science (General) Karlson, Chou Khai Soong Mohd-Noor, Siti Nurfadhlina Nolte, Nadja Tan, Boon Chin CRISPR/dCas9-based systems: Mechanisms and applications in plant sciences |
description |
RNA-guided genomic transcriptional regulation tools, namely clustered regularly interspaced short palindromic repeats interference (CRISPRi) and CRISPR-mediated gene activation (CRISPRa), are a powerful technology for gene functional studies. Deriving from the CRISPR/Cas9 system, both systems consist of a catalytically dead Cas9 (dCas9), a transcriptional effector and a single guide RNA (sgRNA). This type of dCas9 is incapable to cleave DNA but retains its ability to specifically bind to DNA. The binding of the dCas9/sgRNA complex to a target gene results in transcriptional interference. The CRISPR/dCas9 system has been explored as a tool for transcriptional modulation and genome imaging. Despite its potential applications and benefits, the challenges and limitations faced by the CRISPR/dCas9 system include the off-target effects, protospacer adjacent motif (PAM) sequence requirements, efficient delivery methods and the CRISPR/dCas9-interfered crops being labeled as genetically modified organisms in several countries. This review highlights the progression of CRISPR/dCas9 technology as well as its applications and potential challenges in crop improvement.</p> |
format |
Article |
author |
Karlson, Chou Khai Soong Mohd-Noor, Siti Nurfadhlina Nolte, Nadja Tan, Boon Chin |
author_facet |
Karlson, Chou Khai Soong Mohd-Noor, Siti Nurfadhlina Nolte, Nadja Tan, Boon Chin |
author_sort |
Karlson, Chou Khai Soong |
title |
CRISPR/dCas9-based systems: Mechanisms and applications in plant sciences |
title_short |
CRISPR/dCas9-based systems: Mechanisms and applications in plant sciences |
title_full |
CRISPR/dCas9-based systems: Mechanisms and applications in plant sciences |
title_fullStr |
CRISPR/dCas9-based systems: Mechanisms and applications in plant sciences |
title_full_unstemmed |
CRISPR/dCas9-based systems: Mechanisms and applications in plant sciences |
title_sort |
crispr/dcas9-based systems: mechanisms and applications in plant sciences |
publisher |
MDPI |
publishDate |
2021 |
url |
http://eprints.um.edu.my/26413/ |
_version_ |
1735409409482293248 |
score |
13.160551 |