Diagnostic and prognostic value of an immunofluorescent assay for melioidosis
Melioidosis caused by Burkholderia pseudomallei is endemic in southeast Asia. The clinical manifestations range from wound infections to acute septicemia. In some cases, recurrence can also occur following complete recovery. Case fatality rates are high and a major factor is the delay in the culture...
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my.um.eprints.258632021-04-12T02:43:54Z http://eprints.um.edu.my/25863/ Diagnostic and prognostic value of an immunofluorescent assay for melioidosis Vadivelu, Jamuna Puthucheary, Savithri Devi R Medicine Melioidosis caused by Burkholderia pseudomallei is endemic in southeast Asia. The clinical manifestations range from wound infections to acute septicemia. In some cases, recurrence can also occur following complete recovery. Case fatality rates are high and a major factor is the delay in the culture and identification of the bacterium. An immunofluorescent assay (IFAT) using whole-cell antigen for the detection of total antibodies to B. pseudomallei was tested with 650 sera. Using a cut-off value of 1:80, 66 sera from culture-confirmed cases were positive with titers ≥320. In another 523 sera from patients in which no other etiology could be found, 149 (23.4%) were positive. To monitor disease activity, persistence of antibody levels was investigated on 61 serial sera samples collected from 14 other confirmed cases on follow-up visits while on oral maintenance therapy. The IFAT demonstrated a reduction in titers in cases of localized infections, suggesting that either the infection was being resolved or arrested while septicemic patients maintained high IFAT titers on follow-up, suggesting the possibility of continuous sequestration of antigen from an intracellular source. American Society of Tropical Medicine and Hygiene 2000 Article PeerReviewed Vadivelu, Jamuna and Puthucheary, Savithri Devi (2000) Diagnostic and prognostic value of an immunofluorescent assay for melioidosis. The American Journal of Tropical Medicine and Hygiene, 62 (2). pp. 297-300. ISSN 0002-9637 https://doi.org/10.4269/ajtmh.2000.62.297 doi:10.4269/ajtmh.2000.62.297 |
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R Medicine Vadivelu, Jamuna Puthucheary, Savithri Devi Diagnostic and prognostic value of an immunofluorescent assay for melioidosis |
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Melioidosis caused by Burkholderia pseudomallei is endemic in southeast Asia. The clinical manifestations range from wound infections to acute septicemia. In some cases, recurrence can also occur following complete recovery. Case fatality rates are high and a major factor is the delay in the culture and identification of the bacterium. An immunofluorescent assay (IFAT) using whole-cell antigen for the detection of total antibodies to B. pseudomallei was tested with 650 sera. Using a cut-off value of 1:80, 66 sera from culture-confirmed cases were positive with titers ≥320. In another 523 sera from patients in which no other etiology could be found, 149 (23.4%) were positive. To monitor disease activity, persistence of antibody levels was investigated on 61 serial sera samples collected from 14 other confirmed cases on follow-up visits while on oral maintenance therapy. The IFAT demonstrated a reduction in titers in cases of localized infections, suggesting that either the infection was being resolved or arrested while septicemic patients maintained high IFAT titers on follow-up, suggesting the possibility of continuous sequestration of antigen from an intracellular source. |
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Article |
author |
Vadivelu, Jamuna Puthucheary, Savithri Devi |
author_facet |
Vadivelu, Jamuna Puthucheary, Savithri Devi |
author_sort |
Vadivelu, Jamuna |
title |
Diagnostic and prognostic value of an immunofluorescent assay for melioidosis |
title_short |
Diagnostic and prognostic value of an immunofluorescent assay for melioidosis |
title_full |
Diagnostic and prognostic value of an immunofluorescent assay for melioidosis |
title_fullStr |
Diagnostic and prognostic value of an immunofluorescent assay for melioidosis |
title_full_unstemmed |
Diagnostic and prognostic value of an immunofluorescent assay for melioidosis |
title_sort |
diagnostic and prognostic value of an immunofluorescent assay for melioidosis |
publisher |
American Society of Tropical Medicine and Hygiene |
publishDate |
2000 |
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http://eprints.um.edu.my/25863/ https://doi.org/10.4269/ajtmh.2000.62.297 |
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1696976592812638208 |
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13.160551 |