Correlations between serum hdyrogen peroxide level, oxidative damage indices and biochemical markers in end stage renal disease patients

• Main Authors: Nazratun, N., Mahmood Merican, A.A., Tan, S.Y., Ahmad, T.S., Kuppusamy , U.R.
• Format: Article
• Language: English
• Published: 2009
• Subjects: R Medicine
• Online Access: http://eprints.um.edu.my/2504/1/Correlations.PDF; http://eprints.um.edu.my/2504/

Introduction: Hydrogen peroxide is an important intermediate ofendogenous free radical activity which could lead to the generation of more potent free radicals that cause macromolecular damage. An imbalance between the excessive generation of pro-oxidant(free radicals) and insufficient antioxidant defence leads to oxidative stress and this event is marked in end stage renal disease (ESRD) patients. The objective of this study is to assess the correlation between serum hydrogen peroxide level and oxidative damage indices as well as several biochemical markers in ESRD patients. Methods: Fasting blood samples were collected from patients (n=106) attending the University Malaya Medical Centre, Kuala Lumpur. The control subjects mainly comprised healthy blood donors with no known clinical/biochemical abnormality. The serum and plasrna were separated and the levels of hydrogen peroxide, oxidative damage indices (pentosidine, advanced oxidation protein products, malonaldehyde) and biochemical parameters (albumin, creatinine, fenitin, triglyceride, low density lipoproteins and total cholesterol) were estimated according to established rnethods. Results: Levels of hydrogen peroxide and the oxidative damage indices were significantly higher in the patients compared to healthy subjects. A strong positive correlation was present between hydrogen peroxide and the oxidative indices(r>0.80, p<0.001). Hydrogen peroxide was positively (weak) associated with the biochemical parameters except for albumin levels which showed a negative correlation with hydrogen peroxide levels in these patients (r: -0.40, p< 0.01). Conclusion: Serum hydrogen peroxide strongly correlates with oxidative damage indices and could serve as an additional marker of oxidative stress in end stage renal disease.