Rapid and cost-effective antenatal diagnosis of haemoglobin Bart's hydrops foetalis syndrome using a duplex-polymerase chain reaction
Haemoglobin Bart's hydrops foetalis syndrome (--SEA/--SEA) is not compatible with life and contributes to a majority of the hydropic foetuses in the Malaysian Chinese alpha-thalassaemia carriers who possess the 2-alpha-gene deletion in cis (--SEA/alphaalpha). A duplex-PCR which simultaneously a...
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Malaysian Medical Association
2005
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Online Access: | http://eprints.um.edu.my/24542/1/Barts_Hydrops_Foetalis_Syndrome.pdf http://eprints.um.edu.my/24542/ http://www.e-mjm.org/2005/v60n4/Barts_Hydrops_Foetalis_Syndrome.pdf |
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my.um.eprints.245422020-07-20T03:50:17Z http://eprints.um.edu.my/24542/ Rapid and cost-effective antenatal diagnosis of haemoglobin Bart's hydrops foetalis syndrome using a duplex-polymerase chain reaction Wee, Yong Chui Tan, Kim Lian Tan, Peng Chiong Yap, S.F. R Medicine Haemoglobin Bart's hydrops foetalis syndrome (--SEA/--SEA) is not compatible with life and contributes to a majority of the hydropic foetuses in the Malaysian Chinese alpha-thalassaemia carriers who possess the 2-alpha-gene deletion in cis (--SEA/alphaalpha). A duplex-PCR which simultaneously amplifies a normal 136 bp sequence between the psialpha-alpha2-globin genes and a 730 bp Southeast Asian deletion-specific sequence (--SEA) between the psialpha2-theta1-globin genes was established. The duplex-PCR which detects the --SEA deletion in both chromosomes serves as a rapid and cost-effective confirmatory test in the antenatal diagnosis of Haemoglobin Bart's hydrops foetalis syndrome in Malaysia. In addition, the duplex-PCR is simple to perform as both the normal and deletion-specific alpha-globin gene sequences are amplified in the same PCR reaction. Malaysian Medical Association 2005 Article PeerReviewed text en http://eprints.um.edu.my/24542/1/Barts_Hydrops_Foetalis_Syndrome.pdf Wee, Yong Chui and Tan, Kim Lian and Tan, Peng Chiong and Yap, S.F. (2005) Rapid and cost-effective antenatal diagnosis of haemoglobin Bart's hydrops foetalis syndrome using a duplex-polymerase chain reaction. Medical Journal of Malaysia, 60 (4). pp. 447-453. ISSN 0300-5283 http://www.e-mjm.org/2005/v60n4/Barts_Hydrops_Foetalis_Syndrome.pdf |
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R Medicine Wee, Yong Chui Tan, Kim Lian Tan, Peng Chiong Yap, S.F. Rapid and cost-effective antenatal diagnosis of haemoglobin Bart's hydrops foetalis syndrome using a duplex-polymerase chain reaction |
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Haemoglobin Bart's hydrops foetalis syndrome (--SEA/--SEA) is not compatible with life and contributes to a majority of the hydropic foetuses in the Malaysian Chinese alpha-thalassaemia carriers who possess the 2-alpha-gene deletion in cis (--SEA/alphaalpha). A duplex-PCR which simultaneously amplifies a normal 136 bp sequence between the psialpha-alpha2-globin genes and a 730 bp Southeast Asian deletion-specific sequence (--SEA) between the psialpha2-theta1-globin genes was established. The duplex-PCR which detects the --SEA deletion in both chromosomes serves as a rapid and cost-effective confirmatory test in the antenatal diagnosis of Haemoglobin Bart's hydrops foetalis syndrome in Malaysia. In addition, the duplex-PCR is simple to perform as both the normal and deletion-specific alpha-globin gene sequences are amplified in the same PCR reaction. |
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Article |
author |
Wee, Yong Chui Tan, Kim Lian Tan, Peng Chiong Yap, S.F. |
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Wee, Yong Chui Tan, Kim Lian Tan, Peng Chiong Yap, S.F. |
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Wee, Yong Chui |
title |
Rapid and cost-effective antenatal diagnosis of haemoglobin Bart's hydrops foetalis syndrome using a duplex-polymerase chain reaction |
title_short |
Rapid and cost-effective antenatal diagnosis of haemoglobin Bart's hydrops foetalis syndrome using a duplex-polymerase chain reaction |
title_full |
Rapid and cost-effective antenatal diagnosis of haemoglobin Bart's hydrops foetalis syndrome using a duplex-polymerase chain reaction |
title_fullStr |
Rapid and cost-effective antenatal diagnosis of haemoglobin Bart's hydrops foetalis syndrome using a duplex-polymerase chain reaction |
title_full_unstemmed |
Rapid and cost-effective antenatal diagnosis of haemoglobin Bart's hydrops foetalis syndrome using a duplex-polymerase chain reaction |
title_sort |
rapid and cost-effective antenatal diagnosis of haemoglobin bart's hydrops foetalis syndrome using a duplex-polymerase chain reaction |
publisher |
Malaysian Medical Association |
publishDate |
2005 |
url |
http://eprints.um.edu.my/24542/1/Barts_Hydrops_Foetalis_Syndrome.pdf http://eprints.um.edu.my/24542/ http://www.e-mjm.org/2005/v60n4/Barts_Hydrops_Foetalis_Syndrome.pdf |
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