Prediction of signaling pathway induced by solanum nigrum polysaccharide fraction, SN-ppF3 in activating raw 264.7 macrophage cells

• Main Authors: Razali, Faizan Naeem, Shuib, Adawiyah Suriza, Abidin, Nurhayati Zainal
• Format: Article
• Published: Malaysian Society for Biochemistry & Molecular Biology 2019
• Subjects: Q Science (General); QR Microbiology; R Medicine
• Online Access: http://eprints.um.edu.my/23664/; https://drive.google.com/file/d/1nqADLPpKzhdDQI8j0GnUjJv3Hd4c3fiH/view

Previously, the Solanum nigrum polysaccharide fraction, SN-ppF3 was proven to have an immunomodulatory activity by classically activating RAW 264.7 murine macrophage cell line. However, the cellular pathway induced by SN-ppF3 has not to be outlined. In the present study, we predicted the possible cellular pathways induced when macrophage cells were treated with SN-ppF3. The cells were treated with SN-ppF3 for 24 hours and microscopically observed for any morphological due to the treatment. Pinocytosis analysis was carried out to revalidate SN-ppF3 capability as an immunomodulator and also to serve as cytotoxicity evaluation. To outline the signaling pathway induced the cell lysate of 24 hours SN-ppF3-treated macrophage cells were subjected to inflammation analysis through ELISA approach. After the treatment, the morphology of RAW 264.7 cells was obviously altered and pinocytosis activity was significantly increased. In response to the treatment, several phosphorylated proteins such as IκB-α, p38, and NF-κB p65 were significantly up-regulated. Our study suggested that SN-ppF3 treatment could classically activate macrophage through NF-κB pathways which closely similar to the pathway induced by LPS. © 2019 Malaysian Society for Biochemistry and Molecular Biology. All rights reserved.