Production of bio-hydrogen from dairy wastewater using pretreated landfill leachate sludge as an inoculum

• Main Authors: Wong, Yee Meng, Show, Pau Loke, Wu, Ta Yeong, Leong, Hui Yi, Ibrahim, Shaliza, Juan, Joon Ching
• Format: Article
• Published: Elsevier 2019
• Subjects: TA Engineering (General). Civil engineering (General); TP Chemical technology
• Online Access: http://eprints.um.edu.my/23149/; https://doi.org/10.1016/j.jbiosc.2018.07.012

Bio-hydrogen production from wastewater using sludge as inoculum is a sustainable approach for energy production. This study investigated the influence of initial pH and temperature on bio-hydrogen production from dairy wastewater using pretreated landfill leachate sludge (LLS) as an inoculum. The maximum yield of 113.2 ± 2.9 mmol H 2 /g chemical oxygen demand (COD) (12.8 ± 0.3 mmol H 2 /g carbohydrates) was obtained at initial pH 6 and 37 °C. The main products of volatile fatty acids were acetate and butyrate with the ratio of acetate:butyrate was 0.4. At optimum condition, Gibb's free energy was estimated at −40 kJ/mol, whereas the activation enthalpy and entropy were 65 kJ/mol and 0.128 kJ/mol/l, respectively. These thermodynamic quantities suggest that bio-hydrogen production from dairy wastewater using pretreated LLS as inoculum was effective and efficient. In addition, genomic and bioinformatics analyses were performed in this study. © 2018 The Society for Biotechnology, Japan