Alkaloids from Alphonsea elliptica barks and their biological activities
This study was carried out to investigate the phytochemicals from the barks of Alphonsea elliptica. The barks were harvested from Hutan Simpan Sembarong, Kluang, Johor; and extracted using dichloromethane (DCM), hexane and methanol successively. Isolation of dichloromethane crude extract was then pe...
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Journal of Global Pharma Technology
2018
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my.um.eprints.225752019-09-26T03:30:10Z http://eprints.um.edu.my/22575/ Alkaloids from Alphonsea elliptica barks and their biological activities Aldulaimi, Ahmed Kareem Obaid Abdul Azziz, Saripah Salbiah Syed Bakri, Yuhanis Mhd Nafiah, Mohd Azlan Awang, Khalijah Aowda, Saadon Abdulla Litaudon, Marc Hassan, Norazian Mohd Naz, Humera Abbas, Phirdaous Hashim, Yumi Zuhanis Has-Yun Majhool, Alhussein Arkan Q Science (General) QD Chemistry This study was carried out to investigate the phytochemicals from the barks of Alphonsea elliptica. The barks were harvested from Hutan Simpan Sembarong, Kluang, Johor; and extracted using dichloromethane (DCM), hexane and methanol successively. Isolation of dichloromethane crude extract was then performed using silica gel column chromatography and preparative thin layer chromatography (PTLC)techniques. Structural identification were carried out via 1D and 2D NMR, UV, IR, MS and comparison with reported data. Phytochemical study of the barks led tothe isolation of five alkaloid in which two were oxoaporphines; atherospermidine (1) and liriodenine (2),two were dioxoaporphines;cepharadione A (3) and N- methylouregidione (4), and an azafluorenone alkaloid;kinabaline (5). The hexane, DCM, and methanol crude extracts, together with five isolated alkaloids, were tested for theirradical scavenging activity,inhibition of xanthine oxidase activities and cytotoxicity. Atherospermidine and liriodenine possessed antioxidative activities with IC50 value of 20.17 and 10.73, respectively. Atherospermidine, liriodenine, N-methylouregidione and kinabaline showed xanthine oxidase inhibitiory activityof 46.29, 7.66, 42.10 and 50.72 μM,respectively. Meanwhile, atherospermidine, liriodenine, cepharadione A and kinabaline showed cytotoxicity against breast cancer cell line with IC50 89, 86, 79.85 and 62 µg/mL respectively. All the alkaloids tested in this study were isolated for the first time from this species while Cepharadione A is reported for the first time in a species belongs to Alphonsea genus. Journal of Global Pharma Technology 2018 Article PeerReviewed Aldulaimi, Ahmed Kareem Obaid and Abdul Azziz, Saripah Salbiah Syed and Bakri, Yuhanis Mhd and Nafiah, Mohd Azlan and Awang, Khalijah and Aowda, Saadon Abdulla and Litaudon, Marc and Hassan, Norazian Mohd and Naz, Humera and Abbas, Phirdaous and Hashim, Yumi Zuhanis Has-Yun and Majhool, Alhussein Arkan (2018) Alkaloids from Alphonsea elliptica barks and their biological activities. Journal of Global Pharma Technology, 10 (8). pp. 270-275. ISSN 0975-8542 http://www.jgpt.co.in/index.php/jgpt/article/view/1359 |
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Q Science (General) QD Chemistry Aldulaimi, Ahmed Kareem Obaid Abdul Azziz, Saripah Salbiah Syed Bakri, Yuhanis Mhd Nafiah, Mohd Azlan Awang, Khalijah Aowda, Saadon Abdulla Litaudon, Marc Hassan, Norazian Mohd Naz, Humera Abbas, Phirdaous Hashim, Yumi Zuhanis Has-Yun Majhool, Alhussein Arkan Alkaloids from Alphonsea elliptica barks and their biological activities |
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This study was carried out to investigate the phytochemicals from the barks of Alphonsea elliptica. The barks were harvested from Hutan Simpan Sembarong, Kluang, Johor; and extracted using dichloromethane (DCM), hexane and methanol successively. Isolation of dichloromethane crude extract was then performed using silica gel column chromatography and preparative thin layer chromatography (PTLC)techniques. Structural identification were carried out via 1D and 2D NMR, UV, IR, MS and comparison with reported data. Phytochemical study of the barks led tothe isolation of five alkaloid in which two were oxoaporphines; atherospermidine (1) and liriodenine (2),two were dioxoaporphines;cepharadione A (3) and N- methylouregidione (4), and an azafluorenone alkaloid;kinabaline (5). The hexane, DCM, and methanol crude extracts, together with five isolated alkaloids, were tested for theirradical scavenging activity,inhibition of xanthine oxidase activities and cytotoxicity. Atherospermidine and liriodenine possessed antioxidative activities with IC50 value of 20.17 and 10.73, respectively. Atherospermidine, liriodenine, N-methylouregidione and kinabaline showed xanthine oxidase inhibitiory activityof 46.29, 7.66, 42.10 and 50.72 μM,respectively. Meanwhile, atherospermidine, liriodenine, cepharadione A and kinabaline showed cytotoxicity against breast cancer cell line with IC50 89, 86, 79.85 and 62 µg/mL respectively. All the alkaloids tested in this study were isolated for the first time from this species while Cepharadione A is reported for the first time in a species belongs to Alphonsea genus. |
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Article |
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Aldulaimi, Ahmed Kareem Obaid Abdul Azziz, Saripah Salbiah Syed Bakri, Yuhanis Mhd Nafiah, Mohd Azlan Awang, Khalijah Aowda, Saadon Abdulla Litaudon, Marc Hassan, Norazian Mohd Naz, Humera Abbas, Phirdaous Hashim, Yumi Zuhanis Has-Yun Majhool, Alhussein Arkan |
author_facet |
Aldulaimi, Ahmed Kareem Obaid Abdul Azziz, Saripah Salbiah Syed Bakri, Yuhanis Mhd Nafiah, Mohd Azlan Awang, Khalijah Aowda, Saadon Abdulla Litaudon, Marc Hassan, Norazian Mohd Naz, Humera Abbas, Phirdaous Hashim, Yumi Zuhanis Has-Yun Majhool, Alhussein Arkan |
author_sort |
Aldulaimi, Ahmed Kareem Obaid |
title |
Alkaloids from Alphonsea elliptica barks and their biological activities |
title_short |
Alkaloids from Alphonsea elliptica barks and their biological activities |
title_full |
Alkaloids from Alphonsea elliptica barks and their biological activities |
title_fullStr |
Alkaloids from Alphonsea elliptica barks and their biological activities |
title_full_unstemmed |
Alkaloids from Alphonsea elliptica barks and their biological activities |
title_sort |
alkaloids from alphonsea elliptica barks and their biological activities |
publisher |
Journal of Global Pharma Technology |
publishDate |
2018 |
url |
http://eprints.um.edu.my/22575/ http://www.jgpt.co.in/index.php/jgpt/article/view/1359 |
_version_ |
1646210280563146752 |
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13.18916 |