The Effect of Pathogenesis-Related 10 (PR-10) Gene on the Progression of Fusarium Wilt in Musa acuminata cv. Berangan
PR-10 is a member of pathogenesis-related (PR) genes elicited by the plant’s defense mechanism during pathogen attack. Elevated expression of PR-10 upon different pathogen invasions has been observed in many plant species suggesting its role as an anti-bacterial, anti-viral and anti-fungal gene. How...
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my.um.eprints.223702019-09-17T01:41:35Z http://eprints.um.edu.my/22370/ The Effect of Pathogenesis-Related 10 (PR-10) Gene on the Progression of Fusarium Wilt in Musa acuminata cv. Berangan Baharum, Nadiya Akmal Othman, Rofina Yasmin Mohd-Yusuf, Yusmin Tan, Boon Chin Zaidi, Kamilatulhusna Khalid, Norzulaani Q Science (General) QH Natural history PR-10 is a member of pathogenesis-related (PR) genes elicited by the plant’s defense mechanism during pathogen attack. Elevated expression of PR-10 upon different pathogen invasions has been observed in many plant species suggesting its role as an anti-bacterial, anti-viral and anti-fungal gene. However, the effect of PR-10 in mitigating the infection of Fusarium oxysporum f. sp. cubense (Foc), the causal agent of Fusarium wilt in banana has not been reported. In this study, the coding sequences of PR-10 gene isolated from Foc resistant Musa acuminata ssp. malaccensis (MaPR-10) were integrated into a local Foc susceptible commercial banana cultivar, Berangan via co-cultivation of embryogenic cell suspension and Agrobacterium tumefaciens. Out of 17 putative transgenic lines established, 11 of them positively harbored MaPR-10. Among these, Line-19 plantlets showed the most rapid in-vitro propagation and successfully over-expressed the transgene. Following a nursery challenge experiment with a virulent Foc race 4 (CI HIR) isolate, about 30% of Line-19 plants showed a one-week delay in disease progression when compared to the untransformed controls. From the final evaluation performed in the 5th week-post-inoculation, the leaf symptoms index (LSI) and rhizome discoloration index (RDI) of Line-19 was 3.4 and 6.1, respectively, indicating the disease had progressed. The findings of this study enrich the current existing knowledge on the roles of PR-10 in combating fungal disease in plants. Penerbit Universiti Kebangsaan Malaysia 2018 Article PeerReviewed Baharum, Nadiya Akmal and Othman, Rofina Yasmin and Mohd-Yusuf, Yusmin and Tan, Boon Chin and Zaidi, Kamilatulhusna and Khalid, Norzulaani (2018) The Effect of Pathogenesis-Related 10 (PR-10) Gene on the Progression of Fusarium Wilt in Musa acuminata cv. Berangan. Sains Malaysiana, 47 (10). pp. 2291-2300. ISSN 0126-6039 https://doi.org/10.17576/jsm-2018-4710-05 doi:10.17576/jsm-2018-4710-05 |
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Q Science (General) QH Natural history Baharum, Nadiya Akmal Othman, Rofina Yasmin Mohd-Yusuf, Yusmin Tan, Boon Chin Zaidi, Kamilatulhusna Khalid, Norzulaani The Effect of Pathogenesis-Related 10 (PR-10) Gene on the Progression of Fusarium Wilt in Musa acuminata cv. Berangan |
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PR-10 is a member of pathogenesis-related (PR) genes elicited by the plant’s defense mechanism during pathogen attack. Elevated expression of PR-10 upon different pathogen invasions has been observed in many plant species suggesting its role as an anti-bacterial, anti-viral and anti-fungal gene. However, the effect of PR-10 in mitigating the infection of Fusarium oxysporum f. sp. cubense (Foc), the causal agent of Fusarium wilt in banana has not been reported. In this study, the coding sequences of PR-10 gene isolated from Foc resistant Musa acuminata ssp. malaccensis (MaPR-10) were integrated into a local Foc susceptible commercial banana cultivar, Berangan via co-cultivation of embryogenic cell suspension and Agrobacterium tumefaciens. Out of 17 putative transgenic lines established, 11 of them positively harbored MaPR-10. Among these, Line-19 plantlets showed the most rapid in-vitro propagation and successfully over-expressed the transgene. Following a nursery challenge experiment with a virulent Foc race 4 (CI HIR) isolate, about 30% of Line-19 plants showed a one-week delay in disease progression when compared to the untransformed controls. From the final evaluation performed in the 5th week-post-inoculation, the leaf symptoms index (LSI) and rhizome discoloration index (RDI) of Line-19 was 3.4 and 6.1, respectively, indicating the disease had progressed. The findings of this study enrich the current existing knowledge on the roles of PR-10 in combating fungal disease in plants. |
format |
Article |
author |
Baharum, Nadiya Akmal Othman, Rofina Yasmin Mohd-Yusuf, Yusmin Tan, Boon Chin Zaidi, Kamilatulhusna Khalid, Norzulaani |
author_facet |
Baharum, Nadiya Akmal Othman, Rofina Yasmin Mohd-Yusuf, Yusmin Tan, Boon Chin Zaidi, Kamilatulhusna Khalid, Norzulaani |
author_sort |
Baharum, Nadiya Akmal |
title |
The Effect of Pathogenesis-Related 10 (PR-10) Gene on the Progression of Fusarium Wilt in Musa acuminata cv. Berangan |
title_short |
The Effect of Pathogenesis-Related 10 (PR-10) Gene on the Progression of Fusarium Wilt in Musa acuminata cv. Berangan |
title_full |
The Effect of Pathogenesis-Related 10 (PR-10) Gene on the Progression of Fusarium Wilt in Musa acuminata cv. Berangan |
title_fullStr |
The Effect of Pathogenesis-Related 10 (PR-10) Gene on the Progression of Fusarium Wilt in Musa acuminata cv. Berangan |
title_full_unstemmed |
The Effect of Pathogenesis-Related 10 (PR-10) Gene on the Progression of Fusarium Wilt in Musa acuminata cv. Berangan |
title_sort |
effect of pathogenesis-related 10 (pr-10) gene on the progression of fusarium wilt in musa acuminata cv. berangan |
publisher |
Penerbit Universiti Kebangsaan Malaysia |
publishDate |
2018 |
url |
http://eprints.um.edu.my/22370/ https://doi.org/10.17576/jsm-2018-4710-05 |
_version_ |
1646210222188920832 |
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13.18916 |