Interactive effects of temperature and copper toxicity on photosynthetic efficiency and metabolic plasticity in Scenedesmus quadricauda (Chlorophyceae)

Warming and copper (Cu) toxicity are two key abiotic stressors that strongly affect cell growth, photosynthetic rate, and metabolism in microalgae. In this study, a freshwater chlorophyte, Scenedesmus quadricauda, was exposed to various concentrations of copper sulfate (300, 600, and 1000 μM nominal...

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Main Authors: Yong, Wai Kuan, Sim, Kae Shin, Poong, Sze Wan, Wei, Dong, Phang, Siew Moi, Lim, Phaik Eem
Format: Article
Published: Springer Verlag 2018
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Online Access:http://eprints.um.edu.my/20261/
https://doi.org/10.1007/s10811-018-1574-3
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spelling my.um.eprints.202612019-02-12T01:49:31Z http://eprints.um.edu.my/20261/ Interactive effects of temperature and copper toxicity on photosynthetic efficiency and metabolic plasticity in Scenedesmus quadricauda (Chlorophyceae) Yong, Wai Kuan Sim, Kae Shin Poong, Sze Wan Wei, Dong Phang, Siew Moi Lim, Phaik Eem Q Science (General) QH Natural history Warming and copper (Cu) toxicity are two key abiotic stressors that strongly affect cell growth, photosynthetic rate, and metabolism in microalgae. In this study, a freshwater chlorophyte, Scenedesmus quadricauda, was exposed to various concentrations of copper sulfate (300, 600, and 1000 μM nominal concentrations of CuSO4·5H2O) at 25 and 35 °C. The changes in cell density, photosynthetic parameters, in vivo absorption spectra, reactive oxygen species (ROS) levels, and metabolic profile were analyzed. The effects of copper toxicity on the physiology and biochemistry of microalgae were highly dependent on water temperature. The interactive effects of both stressors induced significant impact on the photosynthetic parameters such as maximum quantum yield (Fv/Fm), saturation irradiance (Ek), and non-photochemical quenching (NPQ). Temperature induced significant impact on cell density, Ek and NPQ, while the Cu toxicity significantly affected the Fv/Fm and NPQ. Changes in the in vivo absorption spectra and high levels of reactive oxygen species (ROS) were observed across different treatments. Overall, S. quadricauda adapted to the two abiotic stresses via NPQ and metabolic restructuring. Key metabolites including glycine, proline, hexadecanoic acid, propanoic acid, octadecanoic acid, galactose, lactose, and sucrose were involved in the microalgal response. The synergistic effects of temperature and Cu stresses on microalgae might affect community tolerance and species distribution in the long run. Springer Verlag 2018 Article PeerReviewed Yong, Wai Kuan and Sim, Kae Shin and Poong, Sze Wan and Wei, Dong and Phang, Siew Moi and Lim, Phaik Eem (2018) Interactive effects of temperature and copper toxicity on photosynthetic efficiency and metabolic plasticity in Scenedesmus quadricauda (Chlorophyceae). Journal of Applied Phycology, 30 (6). pp. 3029-3041. ISSN 0921-8971 https://doi.org/10.1007/s10811-018-1574-3 doi:10.1007/s10811-018-1574-3
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic Q Science (General)
QH Natural history
spellingShingle Q Science (General)
QH Natural history
Yong, Wai Kuan
Sim, Kae Shin
Poong, Sze Wan
Wei, Dong
Phang, Siew Moi
Lim, Phaik Eem
Interactive effects of temperature and copper toxicity on photosynthetic efficiency and metabolic plasticity in Scenedesmus quadricauda (Chlorophyceae)
description Warming and copper (Cu) toxicity are two key abiotic stressors that strongly affect cell growth, photosynthetic rate, and metabolism in microalgae. In this study, a freshwater chlorophyte, Scenedesmus quadricauda, was exposed to various concentrations of copper sulfate (300, 600, and 1000 μM nominal concentrations of CuSO4·5H2O) at 25 and 35 °C. The changes in cell density, photosynthetic parameters, in vivo absorption spectra, reactive oxygen species (ROS) levels, and metabolic profile were analyzed. The effects of copper toxicity on the physiology and biochemistry of microalgae were highly dependent on water temperature. The interactive effects of both stressors induced significant impact on the photosynthetic parameters such as maximum quantum yield (Fv/Fm), saturation irradiance (Ek), and non-photochemical quenching (NPQ). Temperature induced significant impact on cell density, Ek and NPQ, while the Cu toxicity significantly affected the Fv/Fm and NPQ. Changes in the in vivo absorption spectra and high levels of reactive oxygen species (ROS) were observed across different treatments. Overall, S. quadricauda adapted to the two abiotic stresses via NPQ and metabolic restructuring. Key metabolites including glycine, proline, hexadecanoic acid, propanoic acid, octadecanoic acid, galactose, lactose, and sucrose were involved in the microalgal response. The synergistic effects of temperature and Cu stresses on microalgae might affect community tolerance and species distribution in the long run.
format Article
author Yong, Wai Kuan
Sim, Kae Shin
Poong, Sze Wan
Wei, Dong
Phang, Siew Moi
Lim, Phaik Eem
author_facet Yong, Wai Kuan
Sim, Kae Shin
Poong, Sze Wan
Wei, Dong
Phang, Siew Moi
Lim, Phaik Eem
author_sort Yong, Wai Kuan
title Interactive effects of temperature and copper toxicity on photosynthetic efficiency and metabolic plasticity in Scenedesmus quadricauda (Chlorophyceae)
title_short Interactive effects of temperature and copper toxicity on photosynthetic efficiency and metabolic plasticity in Scenedesmus quadricauda (Chlorophyceae)
title_full Interactive effects of temperature and copper toxicity on photosynthetic efficiency and metabolic plasticity in Scenedesmus quadricauda (Chlorophyceae)
title_fullStr Interactive effects of temperature and copper toxicity on photosynthetic efficiency and metabolic plasticity in Scenedesmus quadricauda (Chlorophyceae)
title_full_unstemmed Interactive effects of temperature and copper toxicity on photosynthetic efficiency and metabolic plasticity in Scenedesmus quadricauda (Chlorophyceae)
title_sort interactive effects of temperature and copper toxicity on photosynthetic efficiency and metabolic plasticity in scenedesmus quadricauda (chlorophyceae)
publisher Springer Verlag
publishDate 2018
url http://eprints.um.edu.my/20261/
https://doi.org/10.1007/s10811-018-1574-3
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score 13.209306