Cytotoxic Effects of Pinnatane A Extracted from Walsura pinnata (Meliaceae) on Human Liver Cancer Cells
Background: Pinnatane A from the bark of Walsura pinnata was investigated for its anti-cancer properties by analyzing the cytotoxic activities and cell cycle arrest mechanism induced in two different liver cancer cell lines. Methods: A 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide...
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my.um.eprints.201862019-01-28T03:23:46Z http://eprints.um.edu.my/20186/ Cytotoxic Effects of Pinnatane A Extracted from Walsura pinnata (Meliaceae) on Human Liver Cancer Cells Zakaria, Nurhisyam Mahdzir, Mohamad Yusoff, Mahfuzah Arshad, Norhafiza Mohd Awang, Khalijah Nagoor, Noor Hasima Q Science (General) QD Chemistry QH Natural history Background: Pinnatane A from the bark of Walsura pinnata was investigated for its anti-cancer properties by analyzing the cytotoxic activities and cell cycle arrest mechanism induced in two different liver cancer cell lines. Methods: A 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was used to analyze the pinnatane A selectivity in inducing cell death in cancer and normal cells. Various biological assays were carried out to analyze the anti-cancer properties of pinnatane A, such as a live/dead assay for cell death microscopic visualization, cell cycle analysis using propidium iodide (PI) to identify the cell cycle arrest phase, annexin V-fluorescein isothiocyanate (annexin V-FITC)/PI flow cytometry assay to measure percentage of cell populations at different stages of apoptosis and necrosis, and DNA fragmentation assay to verify the late stage of apoptosis. Results: The MTT assay identified pinnatane A prominent dose- and time-dependent cytotoxicity effects in Hep3B and HepG2 cells, with minimal effect on normal cells. The live/dead assay showed significant cell death, while cell cycle analysis showed arrest at the G0/G1 phase in both cell lines. Annexin V-FITC/PI flow cytometry and DNA fragmentation assays identified apoptotic cell death in Hep3B and necrotic cell death in HepG2 cell lines. Conclusions: Pinnatane A has the potential for further development as a chemotherapeutic agent prominently against human liver cells. MDPI 2018 Article PeerReviewed Zakaria, Nurhisyam and Mahdzir, Mohamad and Yusoff, Mahfuzah and Arshad, Norhafiza Mohd and Awang, Khalijah and Nagoor, Noor Hasima (2018) Cytotoxic Effects of Pinnatane A Extracted from Walsura pinnata (Meliaceae) on Human Liver Cancer Cells. Molecules, 23 (11). p. 2733. ISSN 1420-3049 https://doi.org/10.3390/molecules23112733 doi:10.3390/molecules23112733 |
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Q Science (General) QD Chemistry QH Natural history Zakaria, Nurhisyam Mahdzir, Mohamad Yusoff, Mahfuzah Arshad, Norhafiza Mohd Awang, Khalijah Nagoor, Noor Hasima Cytotoxic Effects of Pinnatane A Extracted from Walsura pinnata (Meliaceae) on Human Liver Cancer Cells |
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Background: Pinnatane A from the bark of Walsura pinnata was investigated for its anti-cancer properties by analyzing the cytotoxic activities and cell cycle arrest mechanism induced in two different liver cancer cell lines. Methods: A 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was used to analyze the pinnatane A selectivity in inducing cell death in cancer and normal cells. Various biological assays were carried out to analyze the anti-cancer properties of pinnatane A, such as a live/dead assay for cell death microscopic visualization, cell cycle analysis using propidium iodide (PI) to identify the cell cycle arrest phase, annexin V-fluorescein isothiocyanate (annexin V-FITC)/PI flow cytometry assay to measure percentage of cell populations at different stages of apoptosis and necrosis, and DNA fragmentation assay to verify the late stage of apoptosis. Results: The MTT assay identified pinnatane A prominent dose- and time-dependent cytotoxicity effects in Hep3B and HepG2 cells, with minimal effect on normal cells. The live/dead assay showed significant cell death, while cell cycle analysis showed arrest at the G0/G1 phase in both cell lines. Annexin V-FITC/PI flow cytometry and DNA fragmentation assays identified apoptotic cell death in Hep3B and necrotic cell death in HepG2 cell lines. Conclusions: Pinnatane A has the potential for further development as a chemotherapeutic agent prominently against human liver cells. |
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Article |
author |
Zakaria, Nurhisyam Mahdzir, Mohamad Yusoff, Mahfuzah Arshad, Norhafiza Mohd Awang, Khalijah Nagoor, Noor Hasima |
author_facet |
Zakaria, Nurhisyam Mahdzir, Mohamad Yusoff, Mahfuzah Arshad, Norhafiza Mohd Awang, Khalijah Nagoor, Noor Hasima |
author_sort |
Zakaria, Nurhisyam |
title |
Cytotoxic Effects of Pinnatane A Extracted from Walsura pinnata (Meliaceae) on Human Liver Cancer Cells |
title_short |
Cytotoxic Effects of Pinnatane A Extracted from Walsura pinnata (Meliaceae) on Human Liver Cancer Cells |
title_full |
Cytotoxic Effects of Pinnatane A Extracted from Walsura pinnata (Meliaceae) on Human Liver Cancer Cells |
title_fullStr |
Cytotoxic Effects of Pinnatane A Extracted from Walsura pinnata (Meliaceae) on Human Liver Cancer Cells |
title_full_unstemmed |
Cytotoxic Effects of Pinnatane A Extracted from Walsura pinnata (Meliaceae) on Human Liver Cancer Cells |
title_sort |
cytotoxic effects of pinnatane a extracted from walsura pinnata (meliaceae) on human liver cancer cells |
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MDPI |
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2018 |
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http://eprints.um.edu.my/20186/ https://doi.org/10.3390/molecules23112733 |
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1643691204675633152 |
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13.18916 |