Validation and utilization of an internally controlled multiplex Real-time RT-PCR assay for simultaneous detection of enteroviruses and enterovirus A71 associated with hand foot and mouth disease

• Main Authors: Thanh, T.T., Anh, N.T., Tham, N.T., Van, H.M.T., Sabanathan, S., Qui, P.T., Ngan, T.T., Van, T.T.M., Nguyet, L.A., Ny, N.T.H., Thanh, L.T.M., Ong, Kien Chai, Perera, D., Viet, D.C., Khanh, T.H., Ha, D.Q., Tuan, H.M., Wong, Kum Thong, Hung, N.T., Chau, N.V.V., Thwaites, G., van Doorn, H.R., Van Tan, L.
• Format: Article
• Published: BMC 2015
• Subjects: R Medicine
• Online Access: http://eprints.um.edu.my/19556/; http://dx.doi.org/10.1186/s12985-015-0316-2

Background: Hand foot and mouth disease (HFMD) is a disease of public health importance across the Asia-Pacific region. The disease is caused by enteroviruses (EVs), in particular enterovirus A71 (EV-A71). In EV-A71-associated HFMD, the infection is sometimes associated with severe manifestations in...