Enzymatic electrochemical detection of epidemic-causing Vibrio cholerae with a disposable oligonucleotide-modified screen-printed bisensor coupled to a dry-reagent-based nucleic acid amplification assay

• Main Authors: Yu, C.Y., Ang, G.Y., Chan, Kok Gan, Banga Singh, K.K., Chan, Y.Y.
• Format: Article
• Published: Elsevier 2015
• Subjects: Q Science (General); QH Natural history
• Online Access: http://eprints.um.edu.my/19340/; http://dx.doi.org/10.1016/j.bios.2015.03.048

In this study, we developed a nucleic acid-sensing platform in which a simple, dry-reagent-based nucleic acid amplification assay is combined with a portable multiplex electrochemical genosensor. Preparation of an amplification reaction mix targeting multiple DNA regions of interest is greatly simpl...