Selection of suitable endogenous reference genes for qPCR in kidney and hypothalamus of rats under testosterone influence

• Main Authors: Gholami, K., Loh, S.Y., Salleh, N., Lam, S.K., Hoe, S.Z.
• Format: Article
• Published: Public Library of Science 2017
• Subjects: R Medicine
• Online Access: http://eprints.um.edu.my/19181/; http://dx.doi.org/10.1371/journal.pone.0176368

Real-Time quantitative PCR (qPCR) is the most reliable and accurate technique for analyses of gene expression. Endogenous reference genes are being used to normalize qPCR data even though their expression may vary under different conditions and in different tissues. Nonetheless, verification of expr...