In vitro antiplasmodial and antioxidant activities of bisbenzylisoquinoline alkaloids from Alseodaphne corneri Kosterm
Objective: To study antiplasmodial and antioxidant activities of the isolation of alkaloids from the active dichloromethane extract of Alseodaphne corneri. Methods: Phytochemical studies of the crude extract led to the isolation of six alkaloids using recycle high performance liquid chromatography a...
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my.um.eprints.187242019-03-01T09:13:27Z http://eprints.um.edu.my/18724/ In vitro antiplasmodial and antioxidant activities of bisbenzylisoquinoline alkaloids from Alseodaphne corneri Kosterm Zahari, Azeana Ablat, A. Sivasothy, Y. Mohamad, Jamaludin Choudhary, M.I. Awang, Khalijah Q Science (General) QD Chemistry Objective: To study antiplasmodial and antioxidant activities of the isolation of alkaloids from the active dichloromethane extract of Alseodaphne corneri. Methods: Phytochemical studies of the crude extract led to the isolation of six alkaloids using recycle high performance liquid chromatography and preparative thin layer chromatography. The antiplasmodial activity of the isolated compounds was evaluated using the histidine-rich protein II assay. The isolated alkaloids were also tested for their antioxidant activity using three different assays; DPPH, ferric reducing ability of plasma and metal chelating assays. Results: Malaria infection caused the formation of free radicals which subsequently led to oxidative stress and apoptosis. The antioxidant properties of the alkaloids under investigation revealed that in addition to the antiplasmodial activity, the alkaloids could also prevent oxidative stress. (+)-laurotetanine and (+)-norstephasubine exhibited strong antiplasmodial activities with IC50 values of 0.189 and 0.116 μM, respectively. Conclusions: Interestingly, the two most potent compounds that exhibit antiplasmodial activity also exhibit good antioxidant activities. The crude dichloromethane extract and the isolated compounds exert substantial antiplasmodial and antioxidative activities which in turn suppress oxidative stress and cause less damage to the host. Medknow Publications 2016 Article PeerReviewed Zahari, Azeana and Ablat, A. and Sivasothy, Y. and Mohamad, Jamaludin and Choudhary, M.I. and Awang, Khalijah (2016) In vitro antiplasmodial and antioxidant activities of bisbenzylisoquinoline alkaloids from Alseodaphne corneri Kosterm. Asian Pacific Journal of Tropical Medicine, 9 (4). pp. 328-332. ISSN 1995-7645 http://dx.doi.org/10.1016/j.apjtm.2016.03.008 doi:10.1016/j.apjtm.2016.03.008 |
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Q Science (General) QD Chemistry Zahari, Azeana Ablat, A. Sivasothy, Y. Mohamad, Jamaludin Choudhary, M.I. Awang, Khalijah In vitro antiplasmodial and antioxidant activities of bisbenzylisoquinoline alkaloids from Alseodaphne corneri Kosterm |
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Objective: To study antiplasmodial and antioxidant activities of the isolation of alkaloids from the active dichloromethane extract of Alseodaphne corneri. Methods: Phytochemical studies of the crude extract led to the isolation of six alkaloids using recycle high performance liquid chromatography and preparative thin layer chromatography. The antiplasmodial activity of the isolated compounds was evaluated using the histidine-rich protein II assay. The isolated alkaloids were also tested for their antioxidant activity using three different assays; DPPH, ferric reducing ability of plasma and metal chelating assays. Results: Malaria infection caused the formation of free radicals which subsequently led to oxidative stress and apoptosis. The antioxidant properties of the alkaloids under investigation revealed that in addition to the antiplasmodial activity, the alkaloids could also prevent oxidative stress. (+)-laurotetanine and (+)-norstephasubine exhibited strong antiplasmodial activities with IC50 values of 0.189 and 0.116 μM, respectively. Conclusions: Interestingly, the two most potent compounds that exhibit antiplasmodial activity also exhibit good antioxidant activities. The crude dichloromethane extract and the isolated compounds exert substantial antiplasmodial and antioxidative activities which in turn suppress oxidative stress and cause less damage to the host. |
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Zahari, Azeana Ablat, A. Sivasothy, Y. Mohamad, Jamaludin Choudhary, M.I. Awang, Khalijah |
author_facet |
Zahari, Azeana Ablat, A. Sivasothy, Y. Mohamad, Jamaludin Choudhary, M.I. Awang, Khalijah |
author_sort |
Zahari, Azeana |
title |
In vitro antiplasmodial and antioxidant activities of bisbenzylisoquinoline alkaloids from Alseodaphne corneri Kosterm |
title_short |
In vitro antiplasmodial and antioxidant activities of bisbenzylisoquinoline alkaloids from Alseodaphne corneri Kosterm |
title_full |
In vitro antiplasmodial and antioxidant activities of bisbenzylisoquinoline alkaloids from Alseodaphne corneri Kosterm |
title_fullStr |
In vitro antiplasmodial and antioxidant activities of bisbenzylisoquinoline alkaloids from Alseodaphne corneri Kosterm |
title_full_unstemmed |
In vitro antiplasmodial and antioxidant activities of bisbenzylisoquinoline alkaloids from Alseodaphne corneri Kosterm |
title_sort |
in vitro antiplasmodial and antioxidant activities of bisbenzylisoquinoline alkaloids from alseodaphne corneri kosterm |
publisher |
Medknow Publications |
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2016 |
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http://eprints.um.edu.my/18724/ http://dx.doi.org/10.1016/j.apjtm.2016.03.008 |
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1643690777230966784 |
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13.159267 |