Duplex real-time PCR assay using SYBR Green to detect and quantify Malayan box turtle (Cuora amboinensis) materials in meatballs, burgers, frankfurters and traditional Chinese herbal jelly powder

• Main Authors: Asing, -, Ali, Md. Eaqub, Hamid, Sharifah Bee Abd, Hossain, M., Ahamad, M.N.U., Hossain, S.M.A., Naquiah, N., Zaidul, I.S.M.
• Format: Article
• Published: Taylor & Francis 2016
• Subjects: Q Science (General); RS Pharmacy and materia medica
• Online Access: http://eprints.um.edu.my/18360/; https://doi.org/10.1080/19440049.2016.1236403

The Malayan box turtle (Cuora amboinensis) (MBT) is a vulnerable and protected species widely used in exotic foods and traditional medicines. Currently available polymerase chain reaction (PCR) assays to identify MBT lack automation and involve long targets which break down in processed or denatured...