DNA metabarcoding of insects and allies: an evaluation of primers and pipelines

• Main Authors: Brandon-Mong, G.J., Gan, H.M., Sing, K.W., Lee, P.S., Lim, Phaik Eem, Wilson, J.J.
• Format: Article
• Published: Cambridge University Press 2015
• Subjects: QH Natural history
• Online Access: http://eprints.um.edu.my/16535/; https://doi.org/10.1017/S0007485315000681

Metabarcoding, the coupling of DNA-based species identification and high-throughput sequencing, offers enormous promise for arthropod biodiversity studies but factors such as cost, speed and ease-of-use of bioinformatic pipelines, crucial for making the leapt from demonstration studies to a real-wor...