Synthetic seeds of clitoria ternatea L. for mass propagation and conservation
Synthetic seeds of Clitoria ternatea-L, were successfully created from leaf explants within 3 weeks after germinated on Murashige and Skoog (MS) media. The seeds were initially washed with tap water and teepol, then the seeds were sterilised with 99% (v/v) sodium hypochlorite solution' for 1...
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| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2014
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| Subjects: | |
| Online Access: | http://eprints.um.edu.my/15757/1/0001.pdf http://eprints.um.edu.my/15757/ |
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| Summary: | Synthetic seeds of Clitoria ternatea-L, were successfully created from leaf
explants within 3 weeks after germinated on Murashige and Skoog (MS)
media. The seeds were initially washed with tap water and teepol, then the
seeds were sterilised with 99% (v/v) sodium hypochlorite solution' for 1
minute and rinsed with distilled waterthree times. In a laminar flow cabinet,
the seeds were dipped in 70% (v/v) ethanol for 1 minute and blotted with
steriled tissue, 'The shoot tip explants were encapsulated with 3% alginate
(w/v) which suplemented with various concentrations (0.5-2.5 mg/I) and
combinations ofNAA, BAP and adenine. The optimum concentration for the
formation of encapsulation matrix was 3.0% sodium alginate (NaC6H706).
Encapsulated beads were soaked in 100 mM calcium chloride dehydrate
(CaCI2.2H20) solution for 30 minutes. No suitable beads were formed with
low concentration (1-2%) of sodium alginate. Within 10 minutes soaking in
calciumchloride dehydrate, Clear and.bead formation with no definite shape
was observed. While, within 20 minutes in calcium chloride dehydrate, clear
beads, solid and round in shape was observed, however, inside the bead was
still in liquid condition. In the present study, the rate of germination of
synthetic' seeds were slightly decreased from 90% to 80% after 30 days of
storage at 4°C, To date-this is the first report on synthetic seeds technology
involving Clitoria Ternatea L. as an alternative and supplementary method for
. mass propagation and conservation of this medicinal, |
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