Molecular differentiation of two morphological variants of gracilaria salicornia
Xia in 1986 combined Gracilaria salicornia, G. canaliculata (G. crassa), G. cacalia and G. minor into one species: G. salicornia. Two morphological variants of G. salicornia were collected from different localities in Malaysia. Variant A collected from Morib, Selangor grew on the roots of Avicenni...
Saved in:
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Springer Verlag
2001
|
Subjects: | |
Online Access: | http://eprints.um.edu.my/15364/1/molecular_differentiation_of_two_morphological_variants_of_Gracilaria_salicornia.pdf http://eprints.um.edu.my/15364/ https://doi.org/10.1023/A:1017532624398 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
my.um.eprints.15364 |
---|---|
record_format |
eprints |
spelling |
my.um.eprints.153642021-12-29T01:13:37Z http://eprints.um.edu.my/15364/ Molecular differentiation of two morphological variants of gracilaria salicornia Lim, Phaik Eem Thong, Kwai Lin Phang, Siew Moi QR Microbiology Xia in 1986 combined Gracilaria salicornia, G. canaliculata (G. crassa), G. cacalia and G. minor into one species: G. salicornia. Two morphological variants of G. salicornia were collected from different localities in Malaysia. Variant A collected from Morib, Selangor grew on the roots of Avicennia. The samples showed absence of main axis; segmented constrictions throughout; cylindrical or slightly compressed thalli. Variant B was collected from the mudflats of Tanjung Tuan, growing on rocks, coral or forming mats on the mud. Plants showed absence of main axis; segments were not constricted throughout the plant (if present only slightly articulated at the upper part), branching was dichotomous or irregular; cylindrical or slightly compressed thalli. The technique of Random Amplified Polymorphic DNA analysis (RAPD) was used to investigate molecular characteristics of the two variants. Out of sixty Operon primers that were screened, four primers, OPA 1, OPA 10, OPA 11 and OPK 7 were able to give polymorphism. The fingerprints generated were stable and reproducible on repeated analysis. The DNA fingerprints generated were visually analysed and clustering analysis was carried out using GelCompar 4.0. The matrix of similarities was based on the Dice coefficients (SD) and the cluster analysis was carried out using the unweighted pair group method using arithmetic averages (UPGMA). DNA analysis showed that two primers (OPA 01, CAGGCCCTTC and OPK 07, AGCGAGCAAG) were able to differentiate the two variants. Springer Verlag 2001 Article PeerReviewed application/pdf en http://eprints.um.edu.my/15364/1/molecular_differentiation_of_two_morphological_variants_of_Gracilaria_salicornia.pdf Lim, Phaik Eem and Thong, Kwai Lin and Phang, Siew Moi (2001) Molecular differentiation of two morphological variants of gracilaria salicornia. Journal of Applied Phycology, 13. pp. 335-342. ISSN 0921-8971 https://doi.org/10.1023/A:1017532624398 doi:10.1023/a:1017532624398 |
institution |
Universiti Malaya |
building |
UM Library |
collection |
Institutional Repository |
continent |
Asia |
country |
Malaysia |
content_provider |
Universiti Malaya |
content_source |
UM Research Repository |
url_provider |
http://eprints.um.edu.my/ |
language |
English |
topic |
QR Microbiology |
spellingShingle |
QR Microbiology Lim, Phaik Eem Thong, Kwai Lin Phang, Siew Moi Molecular differentiation of two morphological variants of gracilaria salicornia |
description |
Xia in 1986 combined Gracilaria salicornia, G. canaliculata (G. crassa), G. cacalia and G. minor into one species:
G. salicornia. Two morphological variants of G. salicornia were collected from different localities in Malaysia.
Variant A collected from Morib, Selangor grew on the roots of Avicennia. The samples showed absence of main
axis; segmented constrictions throughout; cylindrical or slightly compressed thalli. Variant B was collected from
the mudflats of Tanjung Tuan, growing on rocks, coral or forming mats on the mud. Plants showed absence of
main axis; segments were not constricted throughout the plant (if present only slightly articulated at the upper
part), branching was dichotomous or irregular; cylindrical or slightly compressed thalli. The technique of Random
Amplified Polymorphic DNA analysis (RAPD) was used to investigate molecular characteristics of the two variants.
Out of sixty Operon primers that were screened, four primers, OPA 1, OPA 10, OPA 11 and OPK 7 were able
to give polymorphism. The fingerprints generated were stable and reproducible on repeated analysis. The DNA
fingerprints generated were visually analysed and clustering analysis was carried out using GelCompar 4.0. The
matrix of similarities was based on the Dice coefficients (SD) and the cluster analysis was carried out using the
unweighted pair group method using arithmetic averages (UPGMA). DNA analysis showed that two primers (OPA
01, CAGGCCCTTC and OPK 07, AGCGAGCAAG) were able to differentiate the two variants. |
format |
Article |
author |
Lim, Phaik Eem Thong, Kwai Lin Phang, Siew Moi |
author_facet |
Lim, Phaik Eem Thong, Kwai Lin Phang, Siew Moi |
author_sort |
Lim, Phaik Eem |
title |
Molecular differentiation of two morphological variants of gracilaria salicornia |
title_short |
Molecular differentiation of two morphological variants of gracilaria salicornia |
title_full |
Molecular differentiation of two morphological variants of gracilaria salicornia |
title_fullStr |
Molecular differentiation of two morphological variants of gracilaria salicornia |
title_full_unstemmed |
Molecular differentiation of two morphological variants of gracilaria salicornia |
title_sort |
molecular differentiation of two morphological variants of gracilaria salicornia |
publisher |
Springer Verlag |
publishDate |
2001 |
url |
http://eprints.um.edu.my/15364/1/molecular_differentiation_of_two_morphological_variants_of_Gracilaria_salicornia.pdf http://eprints.um.edu.my/15364/ https://doi.org/10.1023/A:1017532624398 |
_version_ |
1720980437196603392 |
score |
13.18916 |