Isolation and characterization of Streptomyces spp. from soils
The isolation of forty-eightstrains of actinomycetesfrom eightsoil sampleswas donewith the conventional dilution plate method on Humic acid-vitamin agar (lfVA), Starch Casein agar and Sorenson's agar. FIVAwas found to be the best isolation medium as more strains of actinomycete could be isola...
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Malaysian Society for Molecular Biology and Biotechnology
2001
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my.um.eprints.152912019-02-07T07:08:36Z http://eprints.um.edu.my/15291/ Isolation and characterization of Streptomyces spp. from soils Tan, G.Y. Ho, C.Y. Tan, E.L. Thong, Kwai Lin QR Microbiology The isolation of forty-eightstrains of actinomycetesfrom eightsoil sampleswas donewith the conventional dilution plate method on Humic acid-vitamin agar (lfVA), Starch Casein agar and Sorenson's agar. FIVAwas found to be the best isolation medium as more strains of actinomycete could be isolated. Based on the aerial mycelium colour scheme, three colour groups were recognised. Representative isolates from each colour group were subjected to macrorestriction analysis with the pulsed-field gel electrophoresis (PFGE). The different coloured strainsexhibitedlowlevelsofsimilariry(Dice coefficient,F<0.50).A cetoneextractsoftheisolateswerescreened on mutant yeastsf or Mitogen-Activated Protein Kinase (MAPK) Kinase and MAPK Phosphatasei nhibitor to find an indirect inhibitor of ras. However, no inhibitor was found but seven isolates exhibited toxic effect towards the yeasts. PFGE analysis of these isolates showed that two of isolateswere identical. From the phenotlpic and genotypic characteristics, the two isolates were shown to be of the same strain. Malaysian Society for Molecular Biology and Biotechnology 2001 Article PeerReviewed application/pdf en http://eprints.um.edu.my/15291/1/Isolation_and_characterization_of_Streptomyces_spp_from_soils.pdf Tan, G.Y. and Ho, C.Y. and Tan, E.L. and Thong, Kwai Lin (2001) Isolation and characterization of Streptomyces spp. from soils. Asia Pacific Journal of Molecular Biology and Biotechnology, 9 (2). pp. 139-142. ISSN 0128-7451 http://www.msmbb.my/index.php/archive-issues |
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The isolation of forty-eightstrains of actinomycetesfrom eightsoil sampleswas donewith the conventional
dilution plate method on Humic acid-vitamin agar (lfVA), Starch Casein agar and Sorenson's agar. FIVAwas
found to be the best isolation medium as more strains of actinomycete could be isolated. Based on the aerial
mycelium colour scheme, three colour groups were recognised. Representative isolates from each colour group
were subjected to macrorestriction analysis with the pulsed-field gel electrophoresis (PFGE). The different coloured
strainsexhibitedlowlevelsofsimilariry(Dice coefficient,F<0.50).A cetoneextractsoftheisolateswerescreened
on mutant yeastsf or Mitogen-Activated Protein Kinase (MAPK) Kinase and MAPK Phosphatasei nhibitor to find
an indirect inhibitor of ras. However, no inhibitor was found but seven isolates exhibited toxic effect towards the
yeasts. PFGE analysis of these isolates showed that two of isolateswere identical. From the phenotlpic and genotypic
characteristics, the two isolates were shown to be of the same strain. |
format |
Article |
author |
Tan, G.Y. Ho, C.Y. Tan, E.L. Thong, Kwai Lin |
author_facet |
Tan, G.Y. Ho, C.Y. Tan, E.L. Thong, Kwai Lin |
author_sort |
Tan, G.Y. |
title |
Isolation and characterization of Streptomyces spp. from soils |
title_short |
Isolation and characterization of Streptomyces spp. from soils |
title_full |
Isolation and characterization of Streptomyces spp. from soils |
title_fullStr |
Isolation and characterization of Streptomyces spp. from soils |
title_full_unstemmed |
Isolation and characterization of Streptomyces spp. from soils |
title_sort |
isolation and characterization of streptomyces spp. from soils |
publisher |
Malaysian Society for Molecular Biology and Biotechnology |
publishDate |
2001 |
url |
http://eprints.um.edu.my/15291/1/Isolation_and_characterization_of_Streptomyces_spp_from_soils.pdf http://eprints.um.edu.my/15291/ http://www.msmbb.my/index.php/archive-issues |
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1643690022182846464 |
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13.18916 |