Comparison of fetal bovine serum and human platelet lysate in cultivation and differentiation of dental pulp stem cells into hepatic lineage cells

• Main Authors: Vasanthan, P., Gnanasegaran, N., Govindasamy, V., Abdullah, A.N., Jayaraman, P., Ronald, V.S., Musa, S., Abu Kasim, N.H.
• Format: Article
• Language: English
• Published: Elsevier 2014
• Subjects: RK Dentistry
• Online Access: http://eprints.um.edu.my/11368/1/Comparison_of_fetal_bovine_serum_and_human_platelet_lysate_in_cultivation.pdf; http://eprints.um.edu.my/11368/; http://www.sciencedirect.com/science/article/pii/S1369703X14000989

The scarcity of organs for liver transplant is a major pressure point of liver transplantation. Hence, generating hepatocytes may provide an alternative choice for therapeutic applications. At present, dental pulp stem cell (SCDs) is an emerging source in regenerative medicine. However, existing protocols for cell culture requires fetal bovine serum (FBS) as a nutritional supplement and may carry the risk of transmitting diseases. Therefore, the present study was undertaken to examine the efficacy of human platelet lysate (HPL) as a substitute for FBS in terms of proliferation and differentiation of SCDs into hepatic lineage cells. The result showed that HPL had displayed a superior effect on the proliferation of SCDs. Next, we induced SCDs into hepatic lineage cells which thrived by initiation and followed by maturation into functional hepatocytes for a total of 21 days. We observed that the gene, protein and its functional profile during this differentiation process reiterated in vivo liver development demonstrating a steady down-regulation of early endoderm markers (GATA4, GATA6, SOX17,