Molecular Characterization of Salmonella enterica Serovar Typhimurium Isolated from Human, Food, and Animal Sources in Malaysia
Salmonella Typhimurium is an important nontyphoidal Salmonella serovar associated with foodborne diseases in many parts of the world. This organism is the major causative agent of nontyphoidal salmonellosis in Malaysia. We aimed to investigate the genetic profiles of the strains isolated from clinic...
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my.um.eprints.106452019-11-19T08:18:37Z http://eprints.um.edu.my/10645/ Molecular Characterization of Salmonella enterica Serovar Typhimurium Isolated from Human, Food, and Animal Sources in Malaysia Ngoi, Soo Tein Lindstedt, Bjørn-Arne Watanabe, Haruo Thong, Kwai Lin QR Microbiology Salmonella Typhimurium is an important nontyphoidal Salmonella serovar associated with foodborne diseases in many parts of the world. This organism is the major causative agent of nontyphoidal salmonellosis in Malaysia. We aimed to investigate the genetic profiles of the strains isolated from clinical, zoonotic, and dietary sources in Malaysia using multilocus variable number tandem repeat analysis (MLVA) and pulsed-field gel electrophoresis (PFGE). By focusing on the 5 common variable number tandem repeat (VNTR) loci, we found that PFGE (D = 0.99) was more discriminative than MLVA (D = 0.76). The low MLVA score might be because of a lack of VNTR loci STTR6 (81.0%) and STTR10pl (76.2%). Both subtyping methods suggested that our S. Typhimurium strains were largely endemic with limited genetic variation. Furthermore, we observed that biphasic S. Typhimurium strains were dominant (99%) and multidrug resistance was prevalent (50%) within our sample pool. The most frequently observed phenotypes were resistance to compound sulfonamides (49%), tetracycline (51%), and streptomycin (52%). In this study, we documented the genetic relationship, antimicrobial resistance characteristics, and flagellar-phase dominance among S. Typhimurium strains found in Malaysia. Institute of Infectious Diseases 2013 Article PeerReviewed application/pdf en http://eprints.um.edu.my/10645/1/Ngoi%2C_2013.pdf Ngoi, Soo Tein and Lindstedt, Bjørn-Arne and Watanabe, Haruo and Thong, Kwai Lin (2013) Molecular Characterization of Salmonella enterica Serovar Typhimurium Isolated from Human, Food, and Animal Sources in Malaysia. Japanese Journal of Infectious Diseases, 66 (3). pp. 180-188. ISSN 1344-6304 https://doi.org/10.7883/yoken.66.180 doi:10.7883/yoken.66.180 |
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Salmonella Typhimurium is an important nontyphoidal Salmonella serovar associated with foodborne diseases in many parts of the world. This organism is the major causative agent of nontyphoidal salmonellosis in Malaysia. We aimed to investigate the genetic profiles of the strains isolated from clinical, zoonotic, and dietary sources in Malaysia using multilocus variable number tandem repeat analysis (MLVA) and pulsed-field gel electrophoresis (PFGE). By focusing on the 5 common variable number tandem repeat (VNTR) loci, we found that PFGE (D = 0.99) was more discriminative than MLVA (D = 0.76). The low MLVA score might be because of a lack of VNTR loci STTR6 (81.0%) and STTR10pl (76.2%). Both subtyping methods suggested that our S. Typhimurium strains were largely endemic with limited genetic variation. Furthermore, we observed that biphasic S. Typhimurium strains were dominant (99%) and multidrug resistance was prevalent (50%) within our sample pool. The most frequently observed phenotypes were resistance to compound sulfonamides (49%), tetracycline (51%), and streptomycin (52%). In this study, we documented the genetic relationship, antimicrobial resistance characteristics, and flagellar-phase dominance among S. Typhimurium strains found in Malaysia. |
format |
Article |
author |
Ngoi, Soo Tein Lindstedt, Bjørn-Arne Watanabe, Haruo Thong, Kwai Lin |
author_facet |
Ngoi, Soo Tein Lindstedt, Bjørn-Arne Watanabe, Haruo Thong, Kwai Lin |
author_sort |
Ngoi, Soo Tein |
title |
Molecular Characterization of Salmonella enterica Serovar Typhimurium Isolated from Human, Food, and Animal Sources in Malaysia |
title_short |
Molecular Characterization of Salmonella enterica Serovar Typhimurium Isolated from Human, Food, and Animal Sources in Malaysia |
title_full |
Molecular Characterization of Salmonella enterica Serovar Typhimurium Isolated from Human, Food, and Animal Sources in Malaysia |
title_fullStr |
Molecular Characterization of Salmonella enterica Serovar Typhimurium Isolated from Human, Food, and Animal Sources in Malaysia |
title_full_unstemmed |
Molecular Characterization of Salmonella enterica Serovar Typhimurium Isolated from Human, Food, and Animal Sources in Malaysia |
title_sort |
molecular characterization of salmonella enterica serovar typhimurium isolated from human, food, and animal sources in malaysia |
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Institute of Infectious Diseases |
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2013 |
url |
http://eprints.um.edu.my/10645/1/Ngoi%2C_2013.pdf http://eprints.um.edu.my/10645/ https://doi.org/10.7883/yoken.66.180 |
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1651867346489311232 |
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