Pharmacokinetic profile of a­-tocopherol in three different nano­-emulsion formulations in rats / Murniati Mazri

• Main Author: Mazri, Murniati
• Format: Thesis
• Language: English
• Published: 2006
• Subjects: RM Therapeutics. Pharmacology; RS Pharmacy and materia medica
• Online Access: https://ir.uitm.edu.my/id/eprint/99036/1/99036.PDF; https://ir.uitm.edu.my/id/eprint/99036/

This study was designed to compare the absorption profiles of nano-emulsion containing n-tocopherol formulated with three different oils, i.e. palm oil, soybean oil and VCO in Sprague-Dawley rats. The plasma concentration of n-tocopherol was determined at specific intervals up to 36 hours post administration of formulations with a dose of 30 TIJ/kg of tocopherol. The peak heights were obtained by direct injection of plasma samples into the High Performance Liquid Chromatography (HPLC), after being deproteinized. The total absorption of the n-tocopherol, calculated as the total area under the plasma concentration time curve (AUCo-∞), was markedly greatest for the palm oil, followed by soybean oil and VCO formulation. This study also showed a rapid absorption of n-tocopherol from oils containing long chain fatty acids (palm oil and soybean oil) compared to the medium chain triglyceride oil (VCO). Although apparently AUCo-∞ and Cmax of nano-emulsion of c-tocopherol formulated in palm oil is higher than in soybean oil and in VCO, they were not significantly different when analysed by a one-way analysis of variance (ANOV A). From the results obtained, it can be concluded that palm oil provides better absorption of c-tocopherol when being formulated as nano-emulsion, when compared to other oils (soybean oil and VCO).