Reproducibility comparative research of DNA extraction method performance towards animal based pharmaceutical and food products / Nazhatul Nadiah Md Anuar

• Main Author: Md Anuar, Nazhatul Nadiah
• Format: Thesis
• Language: English
• Published: 2006
• Subjects: DNA. Deoxyribonucleic acids; RM Therapeutics. Pharmacology; RS Pharmacy and materia medica
• Online Access: https://ir.uitm.edu.my/id/eprint/98886/1/98886.PDF; https://ir.uitm.edu.my/id/eprint/98886/

The purpose of this study were to design an optimized DNA extraction method which can produce optimum DNA yield and purity, to design optimized PCR protocol to ensure optimum amplification of porcine target DNA and to link the optimized DNA extraction and PCR method in order to develop a porcine DNA detection system that can be commercialized. This study was began with In house DNA extraction, and was followed by another two commercialize genomic DNA extraction method which were the DNAzol method and the Wizard® Genomic DNA Purification Kit. Subsequent to extraction, DNA quantification was done to check the DNA recovery as well as its purity by spectrophotometry analysis. Besides, running nucleic acid gel electrophoresis also was done to look for the size of the extracted genomic DNA. Next, the efficiency of the optimized In house extraction method was confirmed with polymerase chain reaction (PCR) assay. The sensitivity and specificity of the PCR is important in amplifying the appropriate DNA. Again, nucleic acid gel electrophoresis was run as the final step of corroborating the size of amplified DNA as well as to verify the efficiency of the In house extraction method that has been optimized. This study may help in the halal and haram authentication of food and other products. In conclusion, the In house extraction method that has been optimized is suitable for the detection of porcine DNA traces in various products, particularly in food and health consumer product. The efficiency of the In house extraction method is proven by the successive PCR assay.