Development of assay method for clotrimazole as a sustained release solid dosage form / Sofiyyah Sa’adon
Sustained-release drug delivery systems allow the antifungal agent to reside at the sites of infection for a prolonged period; hence the systems are ideal to be applied topically in order to achieve desirable therapeutic effect. A simple, sensitive, rapid and reproducible assay method has been devel...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
2005
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| Subjects: | |
| Online Access: | https://ir.uitm.edu.my/id/eprint/98650/1/98650.PDF https://ir.uitm.edu.my/id/eprint/98650/ |
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| Summary: | Sustained-release drug delivery systems allow the antifungal agent to reside at the sites of infection for a prolonged period; hence the systems are ideal to be applied topically in order to achieve desirable therapeutic effect. A simple, sensitive, rapid and reproducible assay method has been developed for determination of the antifungal drug, clotrimazole, when it is incorporated with sodium carboxymethylcellulose as the polymer for sustained-release solid dosage form. Chromatographic method was applied by using high performance liquid chromatography (HPLC) equipped with reversed phase octadecylsilica (ODS) C l8 column. The compound was separated with the mobile phase comprised of acetonitrile and 25 mM disodium hydrogen phosphate anhydrous (65:35, v/v) adjusted to pH 8 with o- phosphoric acid. Analysis was run at a flow rate of 1.0 ml/min and the column effluent was monitored at a wavelength of 220 nm. The calibration graph was linear over the concentration range of 10-100 pg/ml with the retention time of 3.74 min. The correlation coefficient of the calibration curves were always >0.999, while the within- day and between-day coefficient of variation were all less than 1%. The developed method has been successfully applied for the determination of clotrimazole from its polymer. |
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