Identification, mutagenicity and antioxidant activities of Malaysian wild mushrooms / Azieana Jinting

Ethnomycological documentation is very scanty in Malaysia and fungi are often ignored not for the lack of potential but because they are more difficult to obtain and a lot less is known about them. Hence, this study was conducted to identify selected wild mushrooms of Malaysia morphologically and th...

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主要作者: Jinting, Azieana
格式: Thesis
語言:English
出版: 2019
在線閱讀:https://ir.uitm.edu.my/id/eprint/89112/1/89112.pdf
https://ir.uitm.edu.my/id/eprint/89112/
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spelling my.uitm.ir.891122024-04-16T02:35:22Z https://ir.uitm.edu.my/id/eprint/89112/ Identification, mutagenicity and antioxidant activities of Malaysian wild mushrooms / Azieana Jinting Jinting, Azieana Ethnomycological documentation is very scanty in Malaysia and fungi are often ignored not for the lack of potential but because they are more difficult to obtain and a lot less is known about them. Hence, this study was conducted to identify selected wild mushrooms of Malaysia morphologically and through molecular biology techniques. Since some mushroom species are poisonous, it is necessary to obtain epidemiological and experimental data on the beneficial effects of the extracts of the species studied. The Ames test was performed with and without metabolic activation by using Salmonella typhimurium bacterial strains TA98 and TA100 to evaluate the mutagenicity of these mushrooms. Previous studies have reported the association of mushrooms with their antioxidant potentials. Spectrophotometric method was used to estimate total phenolic (TPC) and total flavonoid content (TFC) of mushroom extracts. DPPH radical scavenging and ferric reducing antioxidant power (FRAP) assays were used to determine their antioxidant capacity. Mushrooms were identified as Leucoagaricus leucothites, Ascomycota sp. BF135, Xylaria Feejeensis isolate A2S4-D46, Lentinus sajor-caju strain E882B, Lentinus sp. 5-D-3-A(br)-42, Trametes pubescens, Polyporales sp. ‘phlebioid clade’ MS253, Fomitopsis pinicola and Pycnoporus sanguineus. Result indicates that mutagenicity was negative in all strains with and without metabolic activation. Xylaria feejeensis isolate A2S4-D46, Lentinus sp. (2) 5-D-3-A(br)-42 and Pycnoporus sanguineus showed significant differences in their antioxidant properties. The correlation coefficients showed that TPC was strongly correlated with FRAP. This may suggest that phenolic was the major antioxidant components in the mushroom that contributed to the reducing power ability. With more research on these, wild mushrooms could become and ideal sources of nutritional supplements. 2019 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/89112/1/89112.pdf Identification, mutagenicity and antioxidant activities of Malaysian wild mushrooms / Azieana Jinting. (2019) Masters thesis, thesis, Universiti Teknologi MARA (UiTM).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
description Ethnomycological documentation is very scanty in Malaysia and fungi are often ignored not for the lack of potential but because they are more difficult to obtain and a lot less is known about them. Hence, this study was conducted to identify selected wild mushrooms of Malaysia morphologically and through molecular biology techniques. Since some mushroom species are poisonous, it is necessary to obtain epidemiological and experimental data on the beneficial effects of the extracts of the species studied. The Ames test was performed with and without metabolic activation by using Salmonella typhimurium bacterial strains TA98 and TA100 to evaluate the mutagenicity of these mushrooms. Previous studies have reported the association of mushrooms with their antioxidant potentials. Spectrophotometric method was used to estimate total phenolic (TPC) and total flavonoid content (TFC) of mushroom extracts. DPPH radical scavenging and ferric reducing antioxidant power (FRAP) assays were used to determine their antioxidant capacity. Mushrooms were identified as Leucoagaricus leucothites, Ascomycota sp. BF135, Xylaria Feejeensis isolate A2S4-D46, Lentinus sajor-caju strain E882B, Lentinus sp. 5-D-3-A(br)-42, Trametes pubescens, Polyporales sp. ‘phlebioid clade’ MS253, Fomitopsis pinicola and Pycnoporus sanguineus. Result indicates that mutagenicity was negative in all strains with and without metabolic activation. Xylaria feejeensis isolate A2S4-D46, Lentinus sp. (2) 5-D-3-A(br)-42 and Pycnoporus sanguineus showed significant differences in their antioxidant properties. The correlation coefficients showed that TPC was strongly correlated with FRAP. This may suggest that phenolic was the major antioxidant components in the mushroom that contributed to the reducing power ability. With more research on these, wild mushrooms could become and ideal sources of nutritional supplements.
format Thesis
author Jinting, Azieana
spellingShingle Jinting, Azieana
Identification, mutagenicity and antioxidant activities of Malaysian wild mushrooms / Azieana Jinting
author_facet Jinting, Azieana
author_sort Jinting, Azieana
title Identification, mutagenicity and antioxidant activities of Malaysian wild mushrooms / Azieana Jinting
title_short Identification, mutagenicity and antioxidant activities of Malaysian wild mushrooms / Azieana Jinting
title_full Identification, mutagenicity and antioxidant activities of Malaysian wild mushrooms / Azieana Jinting
title_fullStr Identification, mutagenicity and antioxidant activities of Malaysian wild mushrooms / Azieana Jinting
title_full_unstemmed Identification, mutagenicity and antioxidant activities of Malaysian wild mushrooms / Azieana Jinting
title_sort identification, mutagenicity and antioxidant activities of malaysian wild mushrooms / azieana jinting
publishDate 2019
url https://ir.uitm.edu.my/id/eprint/89112/1/89112.pdf
https://ir.uitm.edu.my/id/eprint/89112/
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