DNA extraction of Clarias batrachus from blood / Rozana Abdullah

• Main Author: Abdullah, Rozana
• Format: Student Project
• Language: English
• Published: 2016
• Subjects: Ecosystem health; Catfishes
• Online Access: https://ir.uitm.edu.my/id/eprint/83849/1/83849.PDF; https://ir.uitm.edu.my/id/eprint/83849/

Catfish are the freshwater fish that found in lakes, stream and ponds. Catfishes have a distinctive but extremely varied morphology. To some extent the species are limited in their ecological niches but providing them with basic equipment to dominate benthic habitats in many freshwater ecosystems. This study was conducted to extract the DNA of Clarias batrachus from blood and to determine the size of DNA of C. batrachus. Two sample of C. batrachus were used for blood sample. Purification of total DNA from C. batrachus blood, the processes involved are cell lysis, homogenization, loading, washing, drying and DNA elution that were perform by using DNeasy® Blood and Tissue Kit (Qiagen). By using agarose gel in electrophoresis techniques the DNA molecule size of the species studied can be determine. From the result, the size of extracted genomic DNA from the C. batrachus blood is about 100bp when compared with the DNA marker. The finding of the study is hoped to enhance basic information on C. batrachus for further research.