Isolation of E. coli in chicken bishop noses using microbiology and molecular methods / Siti Nazrina Camalxaman ... [et al.]
Escherichia coli is an organism commonly used as a fecal indicator to assess and monitor the quality of food product to prevent gastrointestinal infection in humans. This study was conducted to detect and isolate the presence of E. coli in poultry products using conventional and molecular detection...
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Faculty of Health Sciences, Universiti Teknologi MARA
2019
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my.uitm.ir.547362021-12-29T23:53:34Z https://ir.uitm.edu.my/id/eprint/54736/ Isolation of E. coli in chicken bishop noses using microbiology and molecular methods / Siti Nazrina Camalxaman ... [et al.] Camalxaman, Siti Nazrina Ithnin, Faizah Haron, Norhisham Rambely, Azlin Sham Mohamed, Emida QR Microbiology Examination and analysis. Composition. Adulteration Escherichia coli is an organism commonly used as a fecal indicator to assess and monitor the quality of food product to prevent gastrointestinal infection in humans. This study was conducted to detect and isolate the presence of E. coli in poultry products using conventional and molecular detection methods. A total of 10 chicken bishop noses were obtained from two supermarkets in Bandar Puncak Alam. Conventional cultural techniques were performed to screen the presence of E. coli. The amplification of the lamB gene from bacterial isolates detected a single band at 309 bp, confirming the presence of E. coli. In total, 6 out 10 chicken bishop noses were positive for E. coli following screening using standard microbiological techniques coupled with PCR. In summary, conventional methods of E. coli detection should be used in conjunction with molecular based tests to provide optimal diagnostics. Additional work is warranted to further characterize the bacterial isolates to determine its clinical significance. Faculty of Health Sciences, Universiti Teknologi MARA 2019-06 Article PeerReviewed text en https://ir.uitm.edu.my/id/eprint/54736/1/54736.pdf ID54736 Camalxaman, Siti Nazrina and Ithnin, Faizah and Haron, Norhisham and Rambely, Azlin Sham and Mohamed, Emida (2019) Isolation of E. coli in chicken bishop noses using microbiology and molecular methods / Siti Nazrina Camalxaman ... [et al.]. Health Scope, 1. pp. 52-56. ISSN 2735-0649 http://healthscopefsk.com/ |
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QR Microbiology Examination and analysis. Composition. Adulteration Camalxaman, Siti Nazrina Ithnin, Faizah Haron, Norhisham Rambely, Azlin Sham Mohamed, Emida Isolation of E. coli in chicken bishop noses using microbiology and molecular methods / Siti Nazrina Camalxaman ... [et al.] |
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Escherichia coli is an organism commonly used as a fecal indicator to assess and monitor the quality of food product to prevent gastrointestinal infection in humans. This study was conducted to detect and isolate the presence of E. coli in poultry products using conventional and molecular detection methods. A total of 10 chicken bishop noses were obtained from two supermarkets in Bandar Puncak Alam. Conventional cultural techniques were performed to screen the presence of E. coli. The amplification of the lamB gene from bacterial isolates detected a single band at 309 bp, confirming the presence of E. coli. In total, 6 out 10 chicken bishop noses were positive for E. coli following screening using standard microbiological techniques coupled with PCR. In summary, conventional methods of E. coli detection should be used in conjunction with molecular based tests to provide optimal diagnostics. Additional work is warranted to further characterize the bacterial isolates to determine its clinical significance. |
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Article |
author |
Camalxaman, Siti Nazrina Ithnin, Faizah Haron, Norhisham Rambely, Azlin Sham Mohamed, Emida |
author_facet |
Camalxaman, Siti Nazrina Ithnin, Faizah Haron, Norhisham Rambely, Azlin Sham Mohamed, Emida |
author_sort |
Camalxaman, Siti Nazrina |
title |
Isolation of E. coli in chicken bishop noses using microbiology and molecular methods / Siti Nazrina Camalxaman ... [et al.] |
title_short |
Isolation of E. coli in chicken bishop noses using microbiology and molecular methods / Siti Nazrina Camalxaman ... [et al.] |
title_full |
Isolation of E. coli in chicken bishop noses using microbiology and molecular methods / Siti Nazrina Camalxaman ... [et al.] |
title_fullStr |
Isolation of E. coli in chicken bishop noses using microbiology and molecular methods / Siti Nazrina Camalxaman ... [et al.] |
title_full_unstemmed |
Isolation of E. coli in chicken bishop noses using microbiology and molecular methods / Siti Nazrina Camalxaman ... [et al.] |
title_sort |
isolation of e. coli in chicken bishop noses using microbiology and molecular methods / siti nazrina camalxaman ... [et al.] |
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Faculty of Health Sciences, Universiti Teknologi MARA |
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2019 |
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https://ir.uitm.edu.my/id/eprint/54736/1/54736.pdf https://ir.uitm.edu.my/id/eprint/54736/ http://healthscopefsk.com/ |
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