Identification of airborne fungi isolated from Faculty of Dentistry in Universiti Teknologi MARA Sungai Buloh Campus / Nurina Hannani Hishammuddin ... [et al.]
Indoor airborne fungi are known to be the contributor to irritative disorders, primarily non-infective diseases such as allergy and asthma. Spores and hyphal fragments of fungi contained in bioaerosols can be breathed in easily and evoke bronchial irritation and allergy and in immunocompromised indi...
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Faculty of Health Sciences, Universiti Teknologi MARA
2019
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my.uitm.ir.546782021-12-22T03:11:34Z https://ir.uitm.edu.my/id/eprint/54678/ Identification of airborne fungi isolated from Faculty of Dentistry in Universiti Teknologi MARA Sungai Buloh Campus / Nurina Hannani Hishammuddin ... [et al.] Sarmin, Nurul ‘Izzah Hishammuddin, Nurina Hannani Yusof, Hartini Public health. Hygiene. Preventive Medicine Transmission of disease Immunologic diseases. Allergy Indoor airborne fungi are known to be the contributor to irritative disorders, primarily non-infective diseases such as allergy and asthma. Spores and hyphal fragments of fungi contained in bioaerosols can be breathed in easily and evoke bronchial irritation and allergy and in immunocompromised individuals, fungi can cause opportunistic infections. Therefore, the present study was undertaken to identify the airborne fungi that have been isolated from the building of Faculty of Dentistry in UiTM Sungai Buloh campus by using macromorphology, micromorphology and also molecular study. Fungi that were kept in 20% glycerol stock were subcultured on Sabouraud dextrose agar (SDA) and were observed for their macromorphological and micromorphological characteristics. The macromorphology of the fungal isolates were observed based on colour, texture and form while the micromorphology were observed for morphological structures such as phialides and metulae. Subsequently, DNA was then extracted and the internal transcribed spacer regions (ITS1-5.8S-ITS2) was amplified using forward primer ITS1 and reverse primer ITS4. One of the amplicons were sent for sequencing and was identified as Penicillium citrinum with 99% sequence similarity. In conclusion, identification of microorganisms isolated from dental building is necessary to minimise cross infection to the patients and healthcare providers. Faculty of Health Sciences, Universiti Teknologi MARA 2019-06 Article PeerReviewed text en https://ir.uitm.edu.my/id/eprint/54678/1/54678.pdf ID54678 Sarmin, Nurul ‘Izzah and Hishammuddin, Nurina Hannani and Yusof, Hartini (2019) Identification of airborne fungi isolated from Faculty of Dentistry in Universiti Teknologi MARA Sungai Buloh Campus / Nurina Hannani Hishammuddin ... [et al.]. Health Scope, 1. pp. 84-88. ISSN 2735-0649 http://healthscopefsk.com/ |
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Public health. Hygiene. Preventive Medicine Transmission of disease Immunologic diseases. Allergy |
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Public health. Hygiene. Preventive Medicine Transmission of disease Immunologic diseases. Allergy Sarmin, Nurul ‘Izzah Hishammuddin, Nurina Hannani Yusof, Hartini Identification of airborne fungi isolated from Faculty of Dentistry in Universiti Teknologi MARA Sungai Buloh Campus / Nurina Hannani Hishammuddin ... [et al.] |
description |
Indoor airborne fungi are known to be the contributor to irritative disorders, primarily non-infective diseases such as allergy and asthma. Spores and hyphal fragments of fungi contained in bioaerosols can be breathed in easily and evoke bronchial irritation and allergy and in immunocompromised individuals, fungi can cause opportunistic infections. Therefore, the present study was undertaken to identify the airborne fungi that have been isolated from the building of Faculty of Dentistry in UiTM Sungai Buloh campus by using macromorphology, micromorphology and also molecular study. Fungi that were kept in 20% glycerol stock were subcultured on Sabouraud dextrose agar (SDA) and were observed for their macromorphological and micromorphological characteristics. The macromorphology of the fungal isolates were observed based on colour, texture and form while the micromorphology were observed for morphological structures such as phialides and metulae. Subsequently, DNA was then extracted and the internal transcribed spacer regions (ITS1-5.8S-ITS2) was amplified using forward primer ITS1 and reverse primer ITS4. One of the amplicons were sent for sequencing and was identified as Penicillium citrinum with 99% sequence similarity. In conclusion, identification of microorganisms isolated from dental building is necessary to minimise cross infection to the patients and healthcare providers. |
format |
Article |
author |
Sarmin, Nurul ‘Izzah Hishammuddin, Nurina Hannani Yusof, Hartini |
author_facet |
Sarmin, Nurul ‘Izzah Hishammuddin, Nurina Hannani Yusof, Hartini |
author_sort |
Sarmin, Nurul ‘Izzah |
title |
Identification of airborne fungi isolated from Faculty of Dentistry in Universiti Teknologi MARA Sungai Buloh Campus / Nurina Hannani Hishammuddin ... [et al.] |
title_short |
Identification of airborne fungi isolated from Faculty of Dentistry in Universiti Teknologi MARA Sungai Buloh Campus / Nurina Hannani Hishammuddin ... [et al.] |
title_full |
Identification of airborne fungi isolated from Faculty of Dentistry in Universiti Teknologi MARA Sungai Buloh Campus / Nurina Hannani Hishammuddin ... [et al.] |
title_fullStr |
Identification of airborne fungi isolated from Faculty of Dentistry in Universiti Teknologi MARA Sungai Buloh Campus / Nurina Hannani Hishammuddin ... [et al.] |
title_full_unstemmed |
Identification of airborne fungi isolated from Faculty of Dentistry in Universiti Teknologi MARA Sungai Buloh Campus / Nurina Hannani Hishammuddin ... [et al.] |
title_sort |
identification of airborne fungi isolated from faculty of dentistry in universiti teknologi mara sungai buloh campus / nurina hannani hishammuddin ... [et al.] |
publisher |
Faculty of Health Sciences, Universiti Teknologi MARA |
publishDate |
2019 |
url |
https://ir.uitm.edu.my/id/eprint/54678/1/54678.pdf https://ir.uitm.edu.my/id/eprint/54678/ http://healthscopefsk.com/ |
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1720439848574124032 |
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13.160551 |