The assessment of efficacy of parkia speciosa leaves methanolic extract as an antimirobial agent / Intan Alyani Normila

• Main Author: Normila, Intan Alyani
• Format: Thesis
• Language: English
• Published: 2017
• Subjects: QR Microbiology; Antibiotic therapy. Antibiotics; Drugs and their actions; Materia medica
• Online Access: https://ir.uitm.edu.my/id/eprint/50140/1/50140.pdf; https://ir.uitm.edu.my/id/eprint/50140/

The multiple drug resistance (MDR) become the biggest concern to the experts in microbiology area as this problem might contribute to failure in the prevalence of bacterial infections. Alternatives were found basically through natural product among the researchers, in order to combat the prevalence of global antimicrobial resistance problem. Parkia speciosa has been reported in the previous study, to have antimicrobial agent properties in the seeds and pods. However, besides seeds, there are more compartments on the plant that been neglected which suggested to contain the same therapeutic properties. Therefore, this study was conducted to assess the antimicrobial activity of Parkia Speciosa leaves extract against Staphylococcus aureus and Escherichia coli. An experimental research design was carried out which involved Antibiotic Susceptibility Test (AST), Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC). The data were analyzed using ANOVA. Methanolic extract of Parkia speciose leaves gives best significant antimicrobial activity against Staphylococcus aureus. While no antimicrobial activity can be observed on Escherichia coli. The MIC and MBC of Staphylococcus aureus were performed in triplicate and showed the significant result (p<0.05). As for MIC, the result showed minimal concentration that can inhibit the bacteria is 31.25 mg/ml. However, the MBC value against Staphylococcus aureus was detected at 500 mg/ml. Based on that, this study revealed that methanolic extract of Parkia speciosa leaves has potential to be used as an antimicrobial agent against gram-positive bacteria Staphylococcus aureus more effectively than gram negative bacteria, Escherichia coli.