Identification of introgressed line of BC1F1 from malaysian rice variety / Nur Aqilah Alias

• Main Author: Alias, Nur Aqilah
• Format: Student Project
• Language: English
• Published: Faculty of Applied Sciences 2015
• Subjects: QR Microbiology; Fungal antigens; Bacteria
• Online Access: http://ir.uitm.edu.my/id/eprint/23907/1/PPb_NUR%20AQILAH%20ALIAS%20AS%20N%2015_5.pdf; http://ir.uitm.edu.my/id/eprint/23907/

Rice blast disease is mostly caused by the fungus, Magnaporthe oryzae. Markerassisted backcrossing (MAB) is one of the reasonable approaches to develop a new resistant variety to confront with this challenge. The objective of the study is to identify the introgressed lines from BC1F1 population. A total of 80 seeds from BC1F1 families were obtained from Agrotechnology and Bioprocess Division, Malaysian Nuclear Agency, Bangi, Selangor, Malaysia. Based on the result there are 82.5% of seeds were successfully germinated meanwhile 17.5% seeds were unsuccessfully germinated. DNA was extracted by following the modified CTAB method. PCR analysis showed a pattern of band generated by using RM413 and RM206 primers. Marker RM 413 was tightly linked with pi-21 demonstrated 4 heterozygous (score as “H”) out of 18 backcrossed plants. RM 413 marker can be further used as a foreground marker selection in future generation in development of rice blast resistant variety in Malaysia.