Isolation of compounds from ethyl acetate crude extracts in stem bark of pithecellobium jiringa / Norshakireen Khairul Shahrom
In this study, ethyl acetate (EA) crude extract of stem bark of Pithecellobium jiringa was subjected to fractional isolation and proceeds with antifungal activity determination. Five different EA fractions were successfully isolated and labelled as FI, F2, F3, F4, and F5. The isolation of EA crud...
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| Main Author: | |
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| Format: | Student Project |
| Language: | English |
| Published: |
Faculty of Applied Sciences
2016
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| Subjects: | |
| Online Access: | http://ir.uitm.edu.my/id/eprint/23082/1/PPb_NORSHAKIREEN%20KHAIRUL%20SHAHROM%20AS%20C%2016_5.PDF http://ir.uitm.edu.my/id/eprint/23082/ |
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| Summary: | In this study, ethyl acetate (EA) crude extract of stem bark of Pithecellobium
jiringa was subjected to fractional isolation and proceeds with antifungal activity
determination. Five different EA fractions were successfully isolated and labelled
as FI, F2, F3, F4, and F5. The isolation of EA crude was achieved by using
column chromatography method and analysed by thin layer chromatographic
technique. Few compounds were identified based on TLC result on TLC plate.
However, the compounds give negative response to antifungal test, which means,
the compounds in the five fractions were not active towards antifungal test on T.
mentagrophyte and C. albicans. There is no inhibition activities reported towards
on these two fungi. The compounds then were been characterized by using GCMS
and IR spectrometer. The major compound is unsaturated fatty acid which is
hexadecanoic acid methyl ester. |
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