In vitro management of ganoderma basal stem rot disease on oil palm with wild basidiomycetes fruiting in grass after rainfall / Nurnadia Natasha Zainal

• Main Author: Zainal, Nurnadia Natasha
• Format: Student Project
• Published: Faculty of Plantation and Agrotechnology 2017
• Subjects: Mushrooms; Wild plants. Wild flowers. Native plants; Pest control and treatment of diseases. Plant protection
• Online Access: http://ir.uitm.edu.my/id/eprint/18910/

Oil palm tree (Elaeis guineensis) are widely grown in tropical countries especially in Malaysia. The widespread of Basal Stem Rot disease that caused by the Ganoderma boninense had been the major problem as it hard to be eliminated and takes years to identify the symptoms. Therefore, the main objective of this studies were to identify wild mushroom species that can inhibit the Ganoderma sp. mycelial growth by isolation of fungi that grow in grass, spore and hyphae identification and determining the pathogenicity activity of isolated fungi against Ganoderma boninense by using the dual culture method. The fruiting body of Ganoderma sp. and different type of wild mushroom were collected and cultured in Potato Dextrose Agar (PDA). The mycelial, hyphae and spore structure of the wild mushroom culture were identified by observing the fungal staining under the microscope. All three wild mushrooms were named by Wild mushroom 1, 2 and 3 as the species name cannot be identified. A five mm diameter of agar disc was taken from each six day-old PDA culture of Ganoderma sp. and the wild mushroom 1, 2 and 3. The agar disc culture of Ganoderma sp. were placed 4 cm opposite site to each agar disc culture of wild mushroom in fresh potato dextrose agar (PDA) plate. All of the fungus was observed growing for six days to determine the growth inhibition of each wild mushrooms fungus against Ganoderma sp. fungus. The result showed that the wild mushroom 3 have the strong pathogenicity with higher percentage of mycelial growth inhibition against Ganoderma sp.