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Detection of sarcocyst in meat from local markets in Selangor: highlighting the use of crude sample in PCR / Azdayanti Muslim … [et al.]

Sarcocystis spp. are obligate intracellular protozoan parasites which cause meat-borne parasitic disease. In Malaysia, sarcocystosis is seen as a potential emerging food-borne zoonosis after a series of large outbreak of human infections. Humans acquire infection either by ingestion of cyst in raw o...

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Main Author: Muslim, Azdayanti
Format: Article
Language:English
Published: Faculty of Medicine 2016
Online Access:http://ir.uitm.edu.my/id/eprint/17136/1/AJ_AZDAYANTI%20MUSLIM%20JCHS%2016.pdf
http://ir.uitm.edu.my/id/eprint/17136/
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spelling my.uitm.ir.171362019-04-25T08:15:25Z http://ir.uitm.edu.my/id/eprint/17136/ Detection of sarcocyst in meat from local markets in Selangor: highlighting the use of crude sample in PCR / Azdayanti Muslim … [et al.] Muslim, Azdayanti Sarcocystis spp. are obligate intracellular protozoan parasites which cause meat-borne parasitic disease. In Malaysia, sarcocystosis is seen as a potential emerging food-borne zoonosis after a series of large outbreak of human infections. Humans acquire infection either by ingestion of cyst in raw or undercooked infected meat or from sporocysts in contaminated food and water. The goal of this study is to identify the presence of sarcocystis parasites in meat of cattle, buffaloes, sheep and goats collected from local markets in Selangor, Malaysia. Methods: A total of 64 skeletal muscles samples (57 cattle, 2 buffaloes, 4 goats and 1 sheep) were collected from local markets. The samples were cut randomly into three pieces, squashed firmly between two glass slides and then examined microscopically for the presence of cysts. Results: Three samples of meat (4.69 %) from cattle (1), buffalo (1) and sheep (1) were found to be positive for cysts. The cysts were confirmed by PCR as sarcocystis sp. Faculty of Medicine 2016 Article PeerReviewed text en http://ir.uitm.edu.my/id/eprint/17136/1/AJ_AZDAYANTI%20MUSLIM%20JCHS%2016.pdf Muslim, Azdayanti (2016) Detection of sarcocyst in meat from local markets in Selangor: highlighting the use of crude sample in PCR / Azdayanti Muslim … [et al.]. Journal of Clinical and Health Sciences, 1 (2). pp. 18-21. ISSN 0127-984X
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
description Sarcocystis spp. are obligate intracellular protozoan parasites which cause meat-borne parasitic disease. In Malaysia, sarcocystosis is seen as a potential emerging food-borne zoonosis after a series of large outbreak of human infections. Humans acquire infection either by ingestion of cyst in raw or undercooked infected meat or from sporocysts in contaminated food and water. The goal of this study is to identify the presence of sarcocystis parasites in meat of cattle, buffaloes, sheep and goats collected from local markets in Selangor, Malaysia. Methods: A total of 64 skeletal muscles samples (57 cattle, 2 buffaloes, 4 goats and 1 sheep) were collected from local markets. The samples were cut randomly into three pieces, squashed firmly between two glass slides and then examined microscopically for the presence of cysts. Results: Three samples of meat (4.69 %) from cattle (1), buffalo (1) and sheep (1) were found to be positive for cysts. The cysts were confirmed by PCR as sarcocystis sp.
format Article
author Muslim, Azdayanti
spellingShingle Muslim, Azdayanti
Detection of sarcocyst in meat from local markets in Selangor: highlighting the use of crude sample in PCR / Azdayanti Muslim … [et al.]
author_facet Muslim, Azdayanti
author_sort Muslim, Azdayanti
title Detection of sarcocyst in meat from local markets in Selangor: highlighting the use of crude sample in PCR / Azdayanti Muslim … [et al.]
title_short Detection of sarcocyst in meat from local markets in Selangor: highlighting the use of crude sample in PCR / Azdayanti Muslim … [et al.]
title_full Detection of sarcocyst in meat from local markets in Selangor: highlighting the use of crude sample in PCR / Azdayanti Muslim … [et al.]
title_fullStr Detection of sarcocyst in meat from local markets in Selangor: highlighting the use of crude sample in PCR / Azdayanti Muslim … [et al.]
title_full_unstemmed Detection of sarcocyst in meat from local markets in Selangor: highlighting the use of crude sample in PCR / Azdayanti Muslim … [et al.]
title_sort detection of sarcocyst in meat from local markets in selangor: highlighting the use of crude sample in pcr / azdayanti muslim … [et al.]
publisher Faculty of Medicine
publishDate 2016
url http://ir.uitm.edu.my/id/eprint/17136/1/AJ_AZDAYANTI%20MUSLIM%20JCHS%2016.pdf
http://ir.uitm.edu.my/id/eprint/17136/
_version_ 1685648900537450496
score 13.214268

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