Effects of nicotine on implantation on sprague dawley rats / Nurulizwani Roni

• Main Author: Roni, Nurulizwani
• Format: Thesis
• Language: English
• Published: 2014
• Subjects: Pharmacopoeias
• Online Access: https://ir.uitm.edu.my/id/eprint/111361/1/111361.PDF; https://ir.uitm.edu.my/id/eprint/111361/

The effect of the nicotine on the number of implantation site was studied in the Sprague Dawley rat. The finding of this experiment resembles the bad effect of nicotine in the cigarettes to the pregnancy women especially on the early stage of embryo's implantation. 0.5 ml of nicotine (5mg/kg body weight) was injected subcutaneously to the pregnant female Sprague Dawley rats according to the group that has been divided. There were three groups involved which were control group (no nicotine induced), nicotine induced group from day one to and day five of pregnancy and nicotine induced group from day one to day nine of pregnancy. On day tenth of pregnancy, laparatomy was done to observed the implantation sites. The implantation sites from nicotine induced groups were compared with the implantation sites of control group. After the pups were delivered, the numbers of pups were compared between control group and nicotine induced group. From result obtained, there were significant different between the nicotine induced groups and the control group. Nicotine induced group from day one until day nine of pregnancy showed the lowest means for both implantation sites and also for numbers of pups delivered. The mean for implantation sites for nicotine induced group from day one until day five of pregnancy were 8.70, meanwhile mean of pups were 5.70. The mean for implantation sites for nicotine induced group for day one until day nine of pregnancy were 6.4 and the mean of pups were 2.70. According to independent t-test from SPSS programme, there were significant different between each of the group when P< 0.05.