Development of PCR based method for the detection of genetic polymorphism of leptin receptor (LEPR) / Nurul Anis Syuhada Zulkifle

• Main Author: Zulkifle, Nurul Anis Syuhada
• Format: Thesis
• Language: English
• Published: 2014
• Subjects: Obesity
• Online Access: https://ir.uitm.edu.my/id/eprint/111228/1/111228.PDF; https://ir.uitm.edu.my/id/eprint/111228/

The problem of overweight and obesity among populations in Asia have been highlighted for more than a decade. As a result, many chronic diseases increase steadily with increasing body mass index (BMI). Overweight and obesity lead to various problematic metabolic effects on blood pressure, cholesterol, triglycerides and insulin resistance. Even though there are many aetiologic factors causing obesity, obesity had been associated with genetic polymorphism leptin receptor gene. Leptin acts through its receptor, leptin receptor (LEPR) which is a single­ transmembrane-domain receptor of the cytokine-receptor family. In this study, Polymerase Chain Reaction (PCR) based method was done to detect the presence of LEPR polymorphism.